计算溶液所需的质量、体积或浓度。
有货
为什么选择此级别
BioReagent,生物染色剂,用于显微镜 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 99 篇同行评审文献引用。
概述
伊红 (Eosin)又称曙红，属人工合成染料中的呫吨类染料，为桃红色或粉红色的粉末，伊红 (醇溶)分子式为C₂₀H₈Br₄O₅，分子量为647.9，伊红最适宜与苏木精配合染色以显示正常或病理组织的形态结构。
伊红染色液 (醇溶, 0.5%)采用特有防腐剂，操作简单，不使用汞、甲醇等有毒试剂，常用于组织切片或培养细胞的染色，染色后细胞浆呈粉红色或红色。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
自备材料：
系列乙醇、4%多聚甲醛、二甲苯或环保浸蜡脱蜡透明液
操作步骤(仅供参考)：
1、样品处理
a) 对于石蜡切片：
① 二甲苯或脱蜡液 (D292661)中脱蜡5-15min。
②更换新鲜的二甲苯或脱蜡液 (D292661)，再脱蜡5-15min。
③无水乙醇5min。
④90%乙醇2min。
⑤70%乙醇2min。
⑥蒸馏水2min。
b) 对于冰冻切片：
蒸馏水2min。
c) 对于培养细胞：
① 用4%多聚甲醛固定10min以上。
② 蒸馏水洗涤2分钟。
③ 更换新鲜的蒸馏水，再洗涤2min。
2、伊红染色：对于上述处理好的样品，伊红染色液染色0.5-5min (根据染色结果和要求调整时间)，如果需要直接观察，可以用70%乙醇洗涤2次；如需脱水、透明后封片按后续步骤进行，70%乙醇洗涤后仍可按照后续步骤进行脱水、透明和封片处理。
注：如果用于免疫组化等染色后的复染，参考上述步骤在其它染色完成后进行伊红染色。
3、脱水、透明、封片或进行其它染色
a) 脱水、透明、封片：
① 95%乙醇脱水2min。
② 更换新鲜的95%乙醇再脱水2min。
③ 无水乙醇脱水2min。
④ 更换新鲜的无水乙醇再脱水2min。
⑤ 二甲苯或脱蜡液 (D292661)透明5min。
⑥ 更换新鲜的二甲苯或脱蜡液 (D292661)，再透明5min。
⑦ 用中性树胶或其它封片剂封片。
⑧ 显微镜下观察，细胞浆呈粉红色或红色。
b) 进行其它染色：
① 如果进行免疫荧光染色或进行Hoechst等荧光染料的染色，在伊红染色液染色后：70％乙醇洗涤2次，每次2min。
② PBS或生理盐水或TBS或TBST等用于免疫染色或荧光染料染色的溶液浸泡5min。
③ 进行免疫荧光染色或其它荧光染料的染色。
注意事项：
1、 如果需要脱水、透明和封片处理，需自备二甲苯或脱蜡液 (D292661)，中性树胶或其它封片剂。
2、 为了您的安全和健康，请穿实验服并戴一次性手套操作。
3、 试剂开封后请尽快使用，以防影响后续实验效果。
Eosin, also known as tetrabromofluorescein, is a type of xanthene dye among synthetic dyes. It appears as a peach-red or pink powder. The molecular formula of eosin (alcohol-soluble) is C₂₀H₈Br₄O₅, with a molecular weight of 647.9. Eosin is most suitable for being used in combination with hematoxylin for staining to display the morphological structure of normal or pathological tissues.
The Eosin Staining Solution (alcohol-soluble, 0.5%) adopts a unique preservative, featuring simple operation and no use of toxic reagents such as mercury and methanol. It is often used for staining tissue sections or cultured cells, and after staining, the cytoplasm shows pink or red color. This reagent is for research use only and not intended for clinical diagnosis or other purposes.
Materials to Be Prepared by Users
A series of ethanol solutions；4% paraformaldehyde；Xylene or environment-friendly wax-immersing, dewaxing and clearing solution
Operating Steps (for Reference Only)
1. Sample Processing
a) For Paraffin Sections:
① Dewax in xylene or dewaxing solution (Cat. No.: D292661) for 5-15 minutes.
② Replace with fresh xylene or dewaxing solution (Cat. No.: D292661) and dewax again for 5-15 minutes.
③ Immerse in absolute ethanol for 5 minutes.
④ Immerse in 90% ethanol for 2 minutes.
⑤ Immerse in 70% ethanol for 2 minutes.
⑥ Immerse in distilled water for 2 minutes.
b) For Frozen Sections:
Immerse in distilled water for 2 minutes.
c) For Cultured Cells:
① Fix with 4% paraformaldehyde for more than 10 minutes.
② Rinse with distilled water for 2 minutes.
③ Replace with fresh distilled water and rinse again for 2 minutes.
2. Eosin Staining
For the samples processed as above, stain with the Eosin Staining Solution for 0.5-5 minutes (adjust the time according to staining results and requirements).
If direct observation is needed, rinse with 70% ethanol twice.
If dehydration, clearing and mounting are required, proceed with the subsequent steps. After rinsing with 70% ethanol, dehydration, clearing and mounting can still be carried out in accordance with the subsequent steps.
Note: If used for counterstaining after staining such as immunohistochemistry, refer to the above steps to perform eosin staining after other staining procedures are completed.
3. Dehydration, Clearing, Mounting or Other Staining Procedures
a) Dehydration, Clearing and Mounting:
① Dehydrate in 95% ethanol for 2 minutes.
② Replace with fresh 95% ethanol and dehydrate again for 2 minutes.
③ Dehydrate in absolute ethanol for 2 minutes.
④ Replace with fresh absolute ethanol and dehydrate again for 2 minutes.
⑤ Clear in xylene or dewaxing solution (Cat. No.: D292661) for 5 minutes.
⑥ Replace with fresh xylene or dewaxing solution (Cat. No.: D292661) and clear again for 5 minutes.
⑦ Mount the section with neutral balsam or other mounting media.
⑧ Observe under a microscope; the cytoplasm should appear pink or red.
b) Other Staining Procedures:
① If immunofluorescence staining or staining with fluorescent dyes such as Hoechst is to be performed, after staining with the Eosin Staining Solution: rinse with 70% ethanol twice, 2 minutes each time.
② Immerse in a solution suitable for immunostaining or fluorescent dye staining (such as PBS, normal saline, TBS or TBST) for 5 minutes.
③ Perform immunofluorescence staining or staining with other fluorescent dyes.
Precautions
1. If dehydration, clearing and mounting are required, users need to prepare xylene or dewaxing solution (Cat. No.: D292661), neutral balsam or other mounting media by themselves.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
3. Please use the reagent as soon as possible after opening to avoid affecting the subsequent experimental results.
规格
别名
曙红染色液 (醇溶, 0.5%)= | 曙红染色液
英文别名
Eosin Y Staining Solution
规格或纯度
BioReagent,生物染色剂,用于显微镜,0.5%
稳定性与储存
Store at room temperature long term (12 months). Store in the dark.
英文名称
Eosin Staining Solution (Alcohol-Soluble, 0.5%)
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
EC号
239-138-3
分子类型
生物试剂/缓冲液
Isomeric SMILES
C1=CC=C2C(=C1)C(=O)OC23C4=CC(=C(C(=C4OC5=C(C(=C(C=C35)Br)O)Br)Br)O)Br
关联CAS
分子量
647.89
Reaxy-Rn
63410
Reaxys-RN link address
https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=63410&ln=
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
熔点
295 °C
安全和危险性（GHS）
象形图
信号词
警告
危险声明
H302: 吞食有害
预防措施声明
P501: 将内容物/容器处理到。。。
P264: 处理后要彻底洗手。
P270: 使用本产品时，请勿进食、饮水或吸烟。
P330: 漱口
P301+P317: 如果被吞咽：请寻求医疗帮助。
WGK Germany
3
RTECS
LM5800000
个人防护装备
Eyeshields,Gloves,type N95 (US),type P1 (EN143) respirator filter
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
通过匹配包装上的批号来查找并下载产品的 COA，每批产品都进行了严格的验证，您可放心使用！
| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ25F1230696 |
分析证书 | E1507828 |
| ZJ26F0636719 |
分析证书 | E1507828 |
| ZJ26F0636718 |
分析证书 | E1507828 |
| ZJ26F0636244 |
分析证书 | E1507828 |
| ZJ25F1230695 |
分析证书 | E1507828 |
技术文档和文章
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
目录： 技术文章
Experimental Judgment and Condition Selection for Visible-Light Catalysis in Organic Synthesis
目录： 技术文章
Reaction Selection in Small-Molecule Synthesis: From Transformation-Type Assessment to Experimental Route Screening
目录： 技术文章
Applications and Comparison of Histological Counterstaining, Collagen Evaluation, and Protein Staining Methods
目录： 技术文章
Eosinophil Morphological Observation: Staining Basis, Identification Points, and Research Validation
目录： 技术文章
此产品的引用文献
溶液计算器
常见问题
什么是「生物染色剂」？
「生物染色剂」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
什么是「BioReagent」？
「BioReagent」表示该产品经过加工和检测，适用于生物和生化应用。规格可能包括内毒素限值、微生物控制、无菌性、低 DNase/RNase/蛋白酶活性，以及在相关情况下的生物活性验证。
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
本产品如何运输？
本产品按常温标准条件运输，无需温控包装。
本产品的 CAS 号、分子式和分子量是多少？
本产品的 CAS 号为 15086-94-9。
如何获取批次专用COA？
每个订单都会随附分析证书（COA），您也可以从账户订单历史中下载。如需订购前的COA，请联系您的客户代表，或使用PDP上的"申请COA"按钮——我们将提供代表性批次的COA。
需要帮助选择级别？
我们的级别选择指南涵盖了目录中所有的变体的纯度、稳定剂状态和应用适用性。
查看 BioReagent 级别指南 → 查看 Biological Stain 级别指南 → 查看 for Microscopy 级别指南 →产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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