本品主要成分为EDTA-2K（乙二胺四乙酸二钾），其化学分子式为C₁₀H₁₄K₂N₂O₈·2H₂O，分子量404.47，CAS号25102-12-9。作为一种离子螯合类抗凝血药，其核心作用机制为与血液中的钙离子结合形成稳定螯合物，从而阻断血液凝固过程。本品用途广泛，可作为络合剂、金属掩蔽剂（用于络合钙、镁及其他金属离子），亦适用于制备抗凝剂、抗凝管及真空抗凝管，配套全血分析仪使用。在实验应用中，本品适用于全血细胞分析（常规用量：1.5 mg～2.2 mg可抗凝1 mL血液），尤其适合血小板计数实验；但因本品会影响血小板聚集功能及凝血因子检测结果，故不适用于凝血象及血小板功能相关检查。
本试剂未经灭菌处理，为10×浓缩剂型，使用前需用蒸馏水或去离子水按实验要求进行稀释。本品仅适用于科研领域，禁止用于临床诊断或其他医疗用途。
操作步骤（仅供参考）
1. 请根据具体实验方案及操作规范进行试剂准备与实验操作；
2. 常规使用时，需将本品进行10倍稀释，推荐用量：取0.2mL EDTA.2K抗凝剂 (10×)，可有效抗凝1.8 mL血液，使用前请确保充分混匀。
注意事项
1. 实验过程中需严格遵守无菌操作规范，避免试剂污染，影响实验结果；
2. 为保障实验人员的安全与健康，操作本品时必须穿戴实验服，并佩戴一次性手套，避免试剂直接接触皮肤、黏膜及破损部位；若不慎接触，需立即用大量清水冲洗接触部位。
This product primarily consists of EDTA-2K (Ethylenediaminetetraacetic Acid Dipotassium Salt), with the chemical formula C₁₀H₁₄K₂N₂O₈·2H₂O, a molecular weight of 404.47, and CAS No. 25102-12-9. As an ion-chelating anticoagulant, its core mechanism of action involves binding with calcium ions in the blood to form stable chelate complexes, thereby blocking the blood coagulation process. This product has a wide range of applications and can serve as a complexing agent, metal masking agent (for complexing calcium, magnesium, and other metal ions), and is also suitable for preparing anticoagulants, anticoagulant tubes, and vacuum anticoagulant tubes for use with whole blood analyzers. In laboratory applications, this product is suitable for complete blood cell analysis (typical dosage: 1.5 mg to 2.2 mg can anticoagulate 1 mL of blood), particularly for platelet count experiments. However, as it affects platelet aggregation function and coagulation factor test results, it is not suitable for coagulation profile tests or platelet function-related examinations.
This reagent is not sterilized and is supplied as a 10× concentrate. It must be diluted with distilled water or deionized water according to experimental requirements prior to use. This product is intended for research use only and is prohibited for clinical diagnosis or other medical purposes.
Operating Procedure (for reference only)
Please prepare the reagent and perform experimental operations according to the specific experimental protocol and standard operating procedures.
For routine use, dilute this product 10-fold. Recommended dosage: Take 0.2 mL of the EDTA-2K anticoagulant (10×), which can effectively anticoagulate 1.8 mL of blood. Ensure thorough mixing before use.
Precautions
Strictly adhere to aseptic techniques during experiments to avoid reagent contamination, which may affect experimental results.
To ensure the safety and health of laboratory personnel, a lab coat must be worn, and disposable gloves must be used when handling this product. Avoid direct contact of the reagent with skin, mucous membranes, or broken areas. In case of accidental contact, immediately rinse the affected area with plenty of clean water.
ZJ26F1040080
危险品化学品经营许可证（带存储）