BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β-淀粉样蛋白(amyloid-β protein，Aβ)级联学说是AD发病机制的主流学说之一。Aβ聚集形成的老年斑是AD的主要病理特征之一，其主要是由 Aβ1-42异常沉积形成。Aβ1-42多肽可聚集形成寡聚体、纤维等多种不同聚合形态。人体内 Aβ最常见的亚型是 Aβ1-40 和 Aβ1-42。在人脑脊液和血中，Aβ1-40分别比 Aβ1-42的含量水平高 10 倍和 1.5倍，Aβ1-42具有更强的毒性，且更容易聚集，从而形成Aβ 沉淀的核心，引发神经毒性作用 。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠β淀粉样蛋白1-42(Aβ1-42)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠β淀粉样蛋白1-42(Aβ1-42)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat Aβ1-42. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat Aβ1-42 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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