BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
凝血因子Ⅱ（FⅡ，纤维蛋白原酶，凝血酶，凝血剂，凝血酶-C，活化的血液凝固因子II，因子IIa，E型凝血酶，β-凝血酶，γ-凝血酶）是一种丝氨酸蛋白酶，这种酶是由F2基因编码。凝血因子Ⅱ在凝血过程中被蛋白裂解形成凝血酶，凝血酶反过来作为一种丝氨酸蛋白酶，将可溶性纤维蛋白原转化为不溶性的纤维蛋白链，并催化许多其他与凝血有关的反应。FⅡ是由被激活的X因子（Xa）对凝血酶的两个位点进行酶切而产生的。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠凝血因子Ⅱ(FⅡ)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠凝血因子Ⅱ(FⅡ)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat FⅡ. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat FⅡ present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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