BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
泛素羧基端酯酶L1，也被称为UCHL1，是由UCHL1基因编码的一种去泛素化酶。它是基因家族的成员，其产物水解泛素的小C末端加合物以产生泛素单体。它的表达对神经元和弥漫性神经内分泌系统的细胞及其肿瘤具有高度特异性。它广泛存在于所有神经元中，在神经元和睾丸/卵巢中特异性表达。已经发现该基因中的多态性（S18Y）与帕金森病风险降低有关。这种多态性已被特别证明具有抗氧化活性。它的另一个潜在保护功能是其稳定单泛素的能力，这是泛素蛋白酶体系统的重要组成部分。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠泛素羧基端酯酶L1(UCHL1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠泛素羧基端酯酶L1(UCHL1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat UCHL1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat UCHL1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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