BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
前列腺素内过氧化物合酶2，也称为环氧合酶2或COX-2，由PTGS2基因编码的酶。它是两种环氧合酶之一,它涉及花生四烯酸向前列腺素H2的转化，前列腺素H2是在炎症中表达的前列环素的重要前体。PTGS2（COX-2）将花生四烯酸（AA）转化为前列腺素内过氧化物 H2。PTGS 是非甾体抗炎药和 PTGS2（COX-2）特异性抑制剂（称为 coxibs）的靶标。PTGS-2 是一种序列同源二聚体。该酶的每个单体都有一个过氧化物酶和一个 PTGS（COX）活性位点。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠环氧合酶2(COX-2)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠环氧合酶2(COX-2)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat COX-2. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat COX-2 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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