计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
组织蛋白酶L(CTSL),由CTSL1基因编码的蛋白质。该基因编码的蛋白质是一种溶酶体半胱氨酸蛋白酶,在细胞内蛋白质分解代谢中起主要作用。其底物包括胶原蛋白和弹性蛋白,以及α-1蛋白酶抑制剂,α-1蛋白酶抑制剂是中性粒细胞弹性蛋白酶活性的主要控制要素。它与几种病理过程有关,包括肌病和心肌缺血中的肌原纤维坏死,以及肾小管对蛋白尿的反应。它是肽酶C1家族的成员,是由二硫键连接的重链和轻链组成的二聚体,两者均由单一蛋白质前体产生。已发现该基因至少有两种编码相同蛋白质的转录变体。它已被证明与胱抑素A相互作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠组织蛋白酶L(CTSL)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠组织蛋白酶L(CTSL)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat CTSL. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat CTSL present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.