计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
组织蛋白酶S(Cath-S),也被称为CTSS,是由CTSS基因编码的蛋白质。该基因存在利用替代多腺苷酸化信号的转录本变异。它是肽酶C1家族的成员,是一种溶酶体半胱氨酸蛋白酶,可参与将抗原蛋白质降解为肽呈递给MHC II类。在肺泡巨噬细胞中,它可以在很宽的pH范围内作为弹性蛋白酶起作用。它在瘙痒和疼痛或伤害感受中起作用。它由抗原呈递细胞表达,包括巨噬细胞、B淋巴细胞、树突状细胞和小胶质细胞。它由一些上皮细胞表达。在用干扰素-γ 刺激后,其表达在人角质形成细胞中显著增加,在银屑病角质形成细胞中由于促炎因子的刺激而升高其表达。相反,皮质胸腺上皮细胞不表达CTSS。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠组织蛋白酶S(Cath-S)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠组织蛋白酶S(Cath-S)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat Cath-S. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat Cath-S present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.