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血管内皮生长因子A（VEGF-A）是一种蛋白质，由VEGFA基因编码的。该基因是血小板衍生生长因子（PDGF）/血管内皮生长因子（VEGF）家族的成员，编码的蛋白质通常以二硫连接的同源二聚体形式出现。该蛋白是一种糖基化的有丝分裂素，特异性地作用于内皮细胞，具有多种作用，包括介导血管通透性增加，诱导血管生成、血管生成和内皮细胞生长，促进细胞迁移，抑制细胞凋亡。编码自由分泌的或与细胞相关的异构体的替代性剪接转录变体已被确定为特征。VEGF-A对血管内皮细胞显示出突出的活性，主要是通过它与内皮细胞膜上突出的VEGFR1和-R2受体的相互作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有大鼠血管内皮生长因子A(VEGF-A)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠血管内皮生长因子A(VEGF-A)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat VEGF-A. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat VEGF-A present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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