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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管内皮生长因子D(VEGF-D),也被称为C-fos诱导生长因子(FIGF),由FIGF基因编码。它在血管生成,淋巴管生成和内皮细胞生长中具有活性,刺激其其增殖和迁移,并对血管通透性有影响。这种分泌的蛋白质经历复杂的蛋白水解成熟,产生多种加工形式,结合和激活VEGFR-2和VEGFR-3受体。它的功能与VEGF-C的功能相似,主要是促进淋巴管的生长,主要通过其受体VEGFR-3作用于淋巴内皮细胞(LECs),促进存活、生长和迁移。可能在胚胎发生过程中静脉和淋巴血管系统的形成中起作用,也可以在维持成人分化的淋巴内皮中起作用。与它相关的疾病包括肛管鳞状细胞癌和肾血管肌脂肪瘤。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠血管内皮生长因子D(VEGF-D)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠血管内皮生长因子D(VEGF-D)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat VEGF-D. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat VEGF-D present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.