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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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谷胱甘肽过氧化物酶 3 (GPx-3),也称为谷胱甘肽过氧化物酶(GPx-P) 或细 胞外谷胱甘肽过氧化物酶,是由 GPX3 基因编码的酶。它属于谷胱甘肽过氧 化物酶家族,在过氧化氢的解毒中起作用。它在其活性位点含有一个硒代半 胱氨酸(Sec) 残基。硒代半胱氨酸由 UGA 密码子编码,通常表示翻译终止。 含 Sec 基因的 3' UTR 有一个共同的茎环结构,即 sec 插入序列(SECIS),它 是识别 UGA 作为 Sec 密码子而不是作为终止信号所必需的。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有大鼠谷胱甘肽过氧化物酶3(GPX3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠谷胱甘肽过氧化物酶3(GPX3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Rat GPX3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Rat GPX3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.