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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
乙酰胆碱受体(缩写为AChR)是一种完整的膜蛋白,对乙酰胆碱的结合作出反应。像其他跨膜受体一样,乙酰胆碱受体根据其对不同分子的相对亲和力和敏感性来分类。尽管所有的乙酰胆碱受体都对乙酰胆碱有反应,但它们对其他分子也有反应。烟碱型乙酰胆碱受体可被箭毒、六甲嘧胺以及蛇和贝类毒液中的毒素(如α-银杏毒素)所阻断。神经肌肉阻断剂等药物可与神经肌肉接头处的烟碱受体可逆地结合,在麻醉中常规使用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠乙酰胆碱受体(AChR)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠乙酰胆碱受体(AChR)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse AChR. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse AChR present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.