计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
低氧诱导因子-1α,也被称为HIF-1α,是由HIF1A基因编码的异源二聚体转录因子缺氧诱导因子1(HIF-1)的亚基。它是一个含有蛋白质的基本螺旋环螺旋PAS结构域,被认为是细胞和发育对缺氧反应的主转录调节剂。通过缺氧或遗传交替引起的HIF1A失调和过度表达与癌症生物学以及许多其他病理生理学密切相关,特别是在血管形成和血管生成,能量代谢,细胞存活和肿瘤侵袭等领域。HIF1A多肽还包含一个核定位信号基序,两个反式激活结构域CTAD和NTAD,以及一个可以抑制CTAD和NTAD转录活性的干预抑制结构域(ID)。它的稳定性、亚细胞定位以及转录活性尤其受氧水平的影响。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠低氧诱导因子-1α(HIF-1α)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠低氧诱导因子-1α(HIF-1α)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse HIF-1α. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse HIF-1α present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.