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泛素硫酯酶OTU1（YOD1）是一种去泛素化酶，属于卵巢肿瘤相关蛋白酶（OTU）超家族成员。YOD1通过特异性切割蛋白质上的泛素链，调控底物蛋白的稳定性与功能，广泛参与细胞信号转导、免疫应答及蛋白质质量控制等关键生物学过程。在炎症调控中，YOD1通过稳定RIPK2促进NOD2信号通路活化，抑制结肠炎发生；在肿瘤领域，YOD1可调节CDK1稳定性驱动三阴性乳腺癌进展，或通过调控β-catenin影响血管内皮-间质转化。此外，YOD1的表达水平受营养状态调控，参与肝脏脂代谢。其功能异常与炎症性肠病、乳腺癌及心血管疾病等密切相关，表明YOD1是潜在的治疗靶点，为相关疾病防治提供了新思路。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠泛素硫酯酶OTU1(YOD1)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠泛素硫酯酶OTU1(YOD1)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse YOD1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse YOD1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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