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葡萄糖-6-磷酸脱氢酶（G6PD或G6PDH）是一种催化化学反应的细胞膜酶。这种酶参与了磷酸戊糖，通过维持辅酶烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的水平向细胞（如红细胞）提供还原性能量的代谢。NADPH反过来维持这些细胞中谷胱甘肽的水平，帮助保护红细胞免受过氧化氢等化合物的氧化损伤。在数量上更重要的是为参与脂肪酸或异戊二烯生物合成的组织，生产NADPH。G6PD在氧化葡萄糖-6-磷酸的同时将NADP+还原成NADPH。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠葡萄糖-6-磷酸脱氢酶(G6PD)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠葡萄糖-6-磷酸脱氢酶(G6PD)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse G6PD. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse G6PD present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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