BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
谷氨酰胺酶（EC 3.5.1.2, glutaminase I, L-glutaminase, glutamine aminohydrolase）是一种氨基水解酶，从谷氨酰胺生成谷氨酸。它有组织特异性的同工酶。它在神经胶质细胞中具有重要作用。它在肝周细胞中表达和活跃，在那里它产生NH3（氨）用于尿素合成，就像谷氨酸脱氢酶一样。它也在肾小管上皮细胞中表达，在那里产生的氨以铵离子形式排泄。铵离子的排泄是肾脏酸碱调节的一个重要机制。在慢性酸中毒期间，肾脏中的谷氨酰胺酶被诱导，从而导致排泄的铵离子数量增加。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有小鼠谷氨酰胺酶(GLNS)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠谷氨酰胺酶(GLNS)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse GLNS. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse GLNS present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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