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转化受体电位阳离子通道亚家族 V 成员 1(TRPV1)又称辣椒素受体和香草素受体 1,是一种蛋白质,由 TRPV1 基因编码。它是第一个被分离出来的瞬时受体电位香草素受体蛋白的成员,而瞬时受体电位蛋白组又是一个亚家族。它的功能是检测和调节体温。此外,TRPV1 还提供烫伤和疼痛的感觉(痛觉)。在初级传入感觉神经元中,它与 TRPA1(一种化学刺激物受体)合作,介导对有害环境刺激的检测。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有小鼠转化受体电位阳离子通道亚家族V成员1(TRPV1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的小鼠转化受体电位阳离子通道亚家族V成员1(TRPV1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Mouse TRPV1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Mouse TRPV1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.