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E3 泛素蛋白连接酶 TRIM21(TRIM21)是一种由 TRIM21 基因编码的蛋白。 该基因的替代剪接转录变体已被描述,但只有一个基因的全长性质被确定。 它在大多数人体组织中都有表达。它是细胞内抗体介导的蛋白分解途径中的 一个细胞内抗体效应物。它能识别 Fc 结构域并与感染细胞的抗体标记的非包 膜病毒上的免疫球蛋白 G、免疫球蛋白 A 和免疫球蛋白 M 结合。通过自体 泛素化或辅助因子的泛素化,它负责将病毒引向蛋白酶体。它本身并不像病 毒外壳和结合的抗体那样在蛋白体中被降解。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人E3泛素蛋白连接酶TRIM21(TRIM21)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人E3泛素蛋白连接酶TRIM21(TRIM21)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TRIM21. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TRIM21 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.