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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
转录信号传导体和激活体5B是一种蛋白质,由STAT5B基因编码。在大多数有完整基因组数据的胎生动物中已经发现了STAT5B的同源物。该基因所编码的蛋白是 STAT家族转录因子的成员。在对细胞因子和生长因子的反应中,STAT家族成员被受体相关激酶磷酸化,然后形成同源或异源二聚体,转入细胞核,在那里充当转录激活剂。这种蛋白介导由各种细胞配体引发的信号转导,如IL2、IL4、CSF1和不同的生长激素。它已被证明参与了不同的生物过程,如TCR信号传导、细胞凋亡、成人乳腺发育和肝脏基因表达的性别二形性。在一小部分急性早幼粒细胞白血病(APML)中,该基因被发现与视黄酸受体-α(RARA)基因融合。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人信号传导转录激活因子5B(STAT5B)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人信号传导转录激活因子5B(STAT5B)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human STAT5B. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human STAT5B present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.