BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
抗凝血酶Ⅲ(AT-Ⅲ)也被称为抗凝血酶。从抗凝血酶I到抗凝血酶IV的名称源于Seegers、Johnson和Fell在20世纪50年代进行的早期研究。抗凝血酶III（AT III）是指血浆中一种能使凝血酶失活的物质。它是使凝血系统的几种酶失活的小蛋白质分子。它是一种由肝脏产生的糖蛋白，由432个氨基酸组成。它包含三个二硫键和总共四个可能的糖基化位点。α-抗凝血酶是在血浆中发现的抗凝血酶的主要形式，并且在其四个糖基化位点的每一个上都具有寡糖。抗凝血酶的次要形式β-抗凝血酶始终未占据单个糖基化位点。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人抗凝血酶Ⅲ(AT-Ⅲ)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人抗凝血酶Ⅲ(AT-Ⅲ)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human AT-Ⅲ. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human AT-Ⅲ present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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