BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
核转录因子 p65(NF-KBp65)又称 p65 或 NFKB3，是一种 REL 相关蛋白，参 与 NF-κB 异源二聚体的形成、核转位和激活。NF-κB 是一个重要的转录因 子复合体，参与所有类型的细胞过程，包括细胞代谢、趋化等。NF-KBp65 的磷酸化和乙酰化是 NF-κB 激活所需的关键。它也被证明可调节免疫反应， 并且 NF-KBp65 的激活与多种类型的癌症呈正相关。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人核转录因子p65(NF-KBp65)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人核转录因子p65(NF-KBp65)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human NF-KBp65. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human NF-KBp65 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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