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泛素特异性肽酶1(USP1),也被称为去泛素化酶1(HUBP),是由USP1基因编码的酶。该基因编码泛素特异性加工蛋白酶家族的成员,该蛋白酶是一种具有His和Cys结构域的去泛素化酶(DUP)。该蛋白质位于细胞质中,并从与泛素融合的前体和泛素化的蛋白质中切割泛素部分。该蛋白质特异性地使Fanconi贫血DNA修复途径中的蛋白质去泛素化。它还通过去泛素化单泛素化PCNA参与PCNA介导的跨损伤合成 (TLS)。它自身几乎没有去泛素化活性,需要与WDR48相互作用才能具有高活性。已经表征了可变剪接变体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人泛素特异性肽酶1(USP1)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人泛素特异性肽酶1(USP1)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human USP1. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human USP1 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.