BioReagent 级 ，适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
组织蛋白酶G（CTSG），是由CTSG基因编码的蛋白质。它是储存在嗜天青颗粒中的糜蛋白酶家族的三种丝氨酸蛋白酶之一，也是肽酶S1蛋白家族的成员。它是一种255-氨基酸-残基蛋白，包括18-残基信号肽，N端的双残基活化肽和羧基末端延伸，它的活性取决于由天冬氨酸、组氨酸和丝氨酸残基组成的催化三元组。它在消除细胞内病原体和分解炎症部位的组织以及抗炎反应中起重要作用。它对革兰氏阴性和阳性细菌发挥广谱抗菌作用。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人组织蛋白酶G(Cath-G)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人组织蛋白酶G(Cath-G)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human Cath-G. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human Cath-G present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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