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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管内皮生长因子C(VEGF-C)是一种蛋白质,是血小板衍生的生长因子/血管内皮生长因子(PDGF/VEGF)家族的成员。由VEGFC基因编码,该基因位于4q34号染色体上。VEGF-C的主要功能是促进淋巴管的生长(淋巴管生成),它主要通过其受体VEGFR-3作用于淋巴内皮细胞(LECs),促进生存、生长和迁移。它由三个结构域组成:中央VEGF同源结构域(VHD)、N端结构域(前肽)和C端结构域(前肽)。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人血管内皮生长因子C(VEGF-C)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人血管内皮生长因子C(VEGF-C)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human VEGF-C. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human VEGF-C present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.