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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
血管内皮生长因子D（VEGF-D）也称C-fos诱导生长因子（FIGF），是一种血管内皮生长因子，是由FIGF基因编码的。该基因编码的蛋白是血小板衍生的生长因子/血管内皮生长因子（PDGF/VEGF）家族的成员，在血管生成、淋巴管生成和内皮细胞生长方面具有活性。这种分泌蛋白经历了复杂的蛋白分解成熟过程，产生了多种加工形式，与VEGFR-2和VEGFR-3受体结合并激活。该蛋白的结构和功能与血管内皮生长因子C相似。 本试剂盒采用双抗体夹心法酶联免疫吸附试验（ELISA）。往预先包被有人血管内皮生长因子D(VEGF-D)捕获抗体的微孔中，依次加入样本、标准品、生物素标记的检测抗体，HRP酶结合物，中间经过温育和洗涤，用底物TMB显色，TMB在过氧化物酶(HRP)的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人血管内皮生长因子D(VEGF-D)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD值），计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human VEGF-D. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human VEGF-D present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.
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