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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
转化生长因子β-3是一种蛋白质,在人类中是由TGFB3基因编码的。它是一种蛋白质,被称为细胞因子,参与细胞分化、胚胎发生和发育。它属于一个称为转化生长因子β超家族的细胞因子大家族,其中包括TGF-β家族、骨形态发生蛋白(BMPs)、生长和分化因子(GDFs)、抑制素和激活素。TGF-β3被认为是在腭部发育过程中调节参与细胞粘附和细胞外基质(ECM)形成的分子。它在控制哺乳动物的肺部发育中也起着至关重要的作用,它还调节该组织中的细胞粘附和ECM形成,并通过调节受伤皮肤中表皮和真皮细胞的运动来控制伤口愈合。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人转化生长因子β3(TGF-β3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人转化生长因子β3(TGF-β3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human TGF-β3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human TGF-β3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.