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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
酰基辅酶 A 合成酶长链家族成员 4,也被称为 ACSL4,是由 ACSL4 基因编 码的酶。该基因编码的蛋白质是长链脂肪酸-辅酶 A 连接酶家族的同工酶。 尽管底物特异性、亚细胞定位和组织分布不同,但该家族的所有同工酶都将 游离的长链脂肪酸转化为脂肪酰基-CoA 酯,从而在脂质生物合成和脂肪酸 降解中发挥关键作用。这种同工酶优先利用花生四烯酸作为底物。这种酶的 缺乏可能导致 智力低下或 Alport 综合征。该基因的可变剪接产生 2 个转录 变体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人酰基辅酶A合成酶长链家族成员4(ACSL4)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人酰基辅酶A合成酶长链家族成员4(ACSL4)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human ACSL4. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human ACSL4 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.