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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
骨成型蛋白 3,又称骨生成素,是一种蛋白质,由 BMP3 基因编码。该基因 所编码的蛋白是转化生长因子β超家族的成员。它和其他骨成型蛋白(BMP) 一样,以其诱导骨和软骨发育的能力而闻名。它是一个二硫连接的同源二聚 体。它对骨密度有负向调节作用。BMP3 在成骨祖细胞的分化中是其他 BMP 的拮抗剂。它在骨折组织中高度表达。BMP3 在许多结肠直肠癌(CRC)病 例中是高甲基化的,因此与其他高甲基化的基因一起,可作为检测早期 CRC 的生物标志物。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人骨成型蛋白3(BMP3)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人骨成型蛋白3(BMP3)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human BMP3. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human BMP3 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.