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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
激肽释放酶10(KLK10)是一种蛋白质,是由KLK10基因编码的。Kallikreins是一个具有不同生理功能的丝氨酸蛋白酶亚组。越来越多的证据表明,许多卡利克蛋白与致癌作用有关,其中一些有可能成为新型癌症和其他疾病的生物标志物。该基因是位于19号染色体上的15个卡利克酶亚家族成员中的一个。其编码的蛋白是分泌的,可能在抑制乳腺癌和前列腺癌的肿瘤发生中发挥作用。该基因的交替剪接导致了编码相同蛋白质的多种转录变体。 本试剂盒采用双抗体夹心法酶联免疫吸附试验(ELISA)。往预先包被有人激肽释放酶10(KLK10)捕获抗体的微孔中,依次加入样本、标准品、生物素标记的检测抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人激肽释放酶10(KLK10)呈正相关。用酶标仪在450nm 波长下测定吸光度(OD值),计算样本浓度。
This kit employs the sandwich enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with a capture antibody specific to Human KLK10. Following this, the sample, standards, a biotin-conjugated detection antibody, and an HRP (horseradish peroxidase)-labeled conjugate are added sequentially to the wells. The procedure involves incubation and wash steps between each addition. For detection, TMB substrate is used. TMB yields a blue color in the presence of the peroxidase activity of HRP. The reaction is then stopped with an acid solution, which converts the blue color to a final yellow color. The intensity of the yellow color is directly proportional to the concentration of Human KLK10 present in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader. Finally, the concentration of the target analyte in the sample is calculated based on the standard curve generated from the standards.