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BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
促黄体激素(LH,也称为促性腺激素,有时也称为促性腺激素)是由垂体前叶的促性腺激素细胞产生的一种激素。它的产生受来自下丘脑的促性腺激素释放激素(GnRH)的调节。LH是一种异型二聚体糖蛋白,每个单体单位是一个糖蛋白分子,一个α亚单位和一个β亚单位组成完整的功能性蛋白。其结构与其他糖蛋白激素、卵泡刺激素(FSH)、甲状腺刺激素(TSH)和人绒毛膜促性腺激素(hCG)类似。该蛋白二聚体包含2个糖肽亚单位(标记为α-和β-亚单位),它们非共价联系。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠促黄体激素(LH)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠促黄体激素(LH)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat LH antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat LH in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.