计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
环磷酸腺苷(cAMP)是在许多生物过程中很重要的第二信使。cAMP 是三磷酸腺苷(ATP)的衍生物,在许多不同的生物体中用于细胞内信号转导,如将胰高血糖素和肾上腺素等不能通过质膜的激素的作用转移到细胞中。它不应与 5'-AMP 激活的蛋白激酶(AMP-activated protein kinase)相混淆。它还参与了蛋白激酶的激活。此外,cAMP 结合并调节离子通道的功能,如 HCN通道和其他一些环核苷酸结合蛋白,如 Epac1 和 RAPGEF2。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有大鼠环磷酸腺苷(cAMP)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的大鼠环磷酸腺苷(cAMP)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Rat cAMP antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Rat cAMP in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.