计算溶液所需的质量、体积或浓度。
BioReagent 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
4-羟基壬烯醛,或4-羟基-2-壬烯醛或4-HNE或HNE,是一种α,β-不饱和的羟基烯醛,由细胞中的脂质过氧化产生。它是在这个过程中形成的主要的α,β-不饱和羟基烯醛。它是一种无色的油。它存在于整个动物组织中,在氧化应激期间,由于应激事件的增加,脂质过氧化连锁反应的增加,它的数量更高。它被认为在细胞信号转导中起着关键作用,在从细胞周期事件到细胞粘附的各种途径中起着关键作用。 本试剂盒采用竞争法酶联免疫吸附试验(ELISA)。往预先包被有人4-羟基壬烯醛(4-HNE)抗原的微孔中,依次加入样本、标准品、Biotin标记的抗体,HRP酶结合物,中间经过温育和洗涤,用底物TMB显色,TMB在过氧化物酶(HRP)的催化下转化成蓝色,并在酸的作用下转化成最终的黄色。颜色的深浅和样本中的人4-羟基壬烯醛(4-HNE)呈负相关。用酶标仪在450nm波长下测定吸光度(OD值),计算样本浓度。
This kit employs the competitive enzyme-linked immunosorbent assay (ELISA) principle. Microplate wells are pre-coated with Human 4-HNE antigen. The sample, standards, a biotin-labeled antibody, and an HRP enzyme conjugate are then added sequentially to the wells. The procedure includes incubation and wash steps between each addition. TMB substrate is used for color development. In the presence of the peroxidase (HRP) enzyme, TMB is catalyzed to produce a blue color, which is then converted into a final stable yellow color upon the addition of an acid stop solution. The intensity of the resulting yellow color is inversely proportional to the concentration of Human 4-HNE in the sample. The optical density (OD) is measured at a wavelength of 450 nm using a microplate reader, and the concentration of the target analyte in the sample is calculated based on the standard curve.