产品介绍
甲醛，又称蚁醛，是一种有机化合物，通常为无色气体，有刺激性气味，对人眼、鼻等有刺激作用。甲醛在工业中有多种用途，室内装修常用的板材、油漆、地毯、壁纸等都含有甲醛，烟叶的不完全燃烧也能释放甲醛。医学上，甲醛还常被用作防腐剂和消毒剂。目前纺织印染助剂中的甲醛超标主要来源于分散剂 MF、含有甲撑磷酸的螯合剂、丙烯酰胺 N -羟甲基助剂 、皮革鞣剂 、锦纶助剂等苯酚及其衍生物的甲醛缩合物。随着国内外生态纺织品标准的陆续颁布，国内纺织品的生态安全问题受到广泛关注。在纺织品助剂行业，甲醛被广泛用作反应剂，以提高助剂在纺织品上的耐久性，如树脂整理剂、固色剂、黏合剂等。
目前检测甲醛常用的方法有分光光度法和色谱法。分光光度计法相对快捷、方便。在日常生活中应用更为广泛，可以有效检测纺织品中甲醛的含量 。
本试剂盒检测原理：甲醛经水吸收后，在 pH 6.0 条件下，与乙酰丙酮作用，在加热条件下，生成稳定的黄色化合物，以分光光度计412 nm 处测定吸光度，在一定浓度范围黄色化合物的吸光度与甲醛浓度呈线性关系，进而可计算出样品中甲醛的浓度。主要用于室内空气、纺织品等释放的甲醛的检测。该试剂盒仅用于科研领域，不适用于临床诊断或其他用途。
本试剂盒可采用分光光度法进行检测，在样品体积为1 mL 时，检测在0-20 μg/mL 浓度范围内有良好的线性关系 。
组分货号 | 组分名称 | 物理外观 | 50 T | 储存 | 单次测试使用量 |
| F1522035A | 甲醛标准品 (100 mg/L) | 液体 | 3.0 mL | 2-8 ℃. 避光 | 按需 |
| | F1522035B | 乙酰丙酮显色液 | 液体 | 50.0 mL | 2-8 ℃. 避光 | 1.0 mL |
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使用方法
1. 样品准备：
1）室内空气采用相关仪器进行吸收后直接检测。
2）称取一定质量的剪碎的纺织品或处理后的食品用蒸馏水在 40℃条件下振荡浸泡1小时后 用上清液直接检测；如浓度较低，可加大样品的加入量。
2. 配制系列甲醛标准品
将甲醛标准品 (100 mg/L)用蒸馏水稀释至20 μg/mL甲醛标准液，再按下表进行操作，依次稀释。
加入物 (mL) | 1 | 2 | 3 | 4 | 5 | 6 | 甲醛标准液 (20 μg/mL) | 0.05 | 0.2 | 0.35 | 0.5 | 0.75 | 1 | 蒸馏水 | 0.95 | 0.8 | 0.65 | 0.5 | 0.25 | 0 | 相当于甲醛浓度 (μg/mL) | 1 | 4 | 7 | 10 | 15 | 20 |
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3. 加样
按照下表设置空白管、标准管、测定管，溶液应按照顺序依次加入，并注意避免产生气泡。如果样品中的甲醛含量过高，可以减少样品用量或适当稀释后再进行测定，样品的检测最好能设置 2 平行管，求平均值。
加入物 (mL) | 空白管 | 标准管 | 测定管 | 蒸馏水 | 1 | - | - | 甲醛标准液 (1-6) | - | 1 | - | 待测样品 | - | - | 1 | 乙酰丙酮显色液 | 1 | 1 | 1 |
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4. 测定：
立即混匀，40℃水浴 30 min，取出后静置 10 min 冷却至室温。以空白管调零，比色杯光径1 cm，以分光光度计测定 412 nm处系列标准管测定管的吸光度。
注意事项
1) 显色反应可提高反应温度，可适当减少反应时间，有资料提示可沸水浴 3 min。
2) 试剂如不慎溅入眼、口、皮肤等部位，请立即用清水冲洗。
3) 显色后，溶液若呈浑浊状，需经 3000-5000 rpm 离心15 min 后取上清液测定，如果离心后仍浑浊，建议将样品稀释后重新测定。
4) 为了您的安全和健康，请穿实验服并戴一次性手套操作。
5) 试剂开封后请尽快使用，以防影响后续实验效果。
Formaldehyde, also known as methanal, is an organic compound that typically exists as a colorless gas with a pungent odor, irritating the eyes and nose. Formaldehyde has various industrial applications. Materials commonly used in interior decoration, such as wood panels, paints, carpets, and wallpapers, contain formaldehyde, and the incomplete combustion of tobacco can also release formaldehyde. In medicine, formaldehyde is often used as a preservative and disinfectant.
Currently, excessive formaldehyde levels in textile printing and dyeing auxiliaries primarily originate from dispersing agents like MF, chelating agents containing methylene phosphonic acid, acrylamide N-methylol auxiliaries, leather tanning agents, nylon auxiliaries, and formaldehyde condensation products of phenol and its derivatives. With the successive introduction of ecological textile standards both domestically and internationally, the ecological safety of domestic textiles has garnered widespread attention. In the textile auxiliaries industry, formaldehyde is widely used as a reactant to enhance the durability of auxiliaries on textiles, such as in resin finishing agents, fixing agents, and adhesives.
Common methods for detecting formaldehyde include spectrophotometry and chromatography. Spectrophotometry is relatively quick and convenient, making it more widely used in daily applications. It can effectively detect formaldehyde content in textile samples.
The detection principle of this kit is based on the absorption of formaldehyde by water, followed by its reaction with acetylacetone under pH 6.0 conditions. Upon heating, a stable yellow compound is formed. The absorbance of this yellow compound is measured using a spectrophotometer at 412 nm. Within a certain concentration range, the absorbance of the yellow compound is linearly related to the formaldehyde concentration, allowing for the calculation of formaldehyde concentration in the sample. This product is primarily used for detecting formaldehyde released from indoor air, textiles, and similar sources. It is intended for research purposes only and is not suitable for clinical diagnosis or other applications.
ZJ26F0838524
危险品化学品经营许可证（带存储）