Enzyme-Coupled Colorimetric Reaction

Functional Roles of Auxiliary Enzymes in Enzyme-Coupled Colorimetric Reactions: Mutarotase, Oxidases, Peroxidases, and Dehydrogenases

The accuracy of enzyme-coupled colorimetric reactions depends on whether the roles of auxiliary enzymes are clearly defined. Mutarotase accelerates the equilibrium of sugar anomers. Oxidases and dehydrogenases convert analytes into intermediate signals such as H₂O₂ or NAD(P)H. Peroxidases then ...