Western Blotting with Fluorescently Labeled Antibodies
For western blotting, incubate the membrane with primary antibody diluted in 1×TBST (0.1% Tween®20) containing 5% w/v BSA or nonfat dry milk overnight at 4°C on a shaker. Please refer to the primary antibody product webpage for recommended primary antibody dilution buffer and antibody dilution.
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