葡萄糖-6-磷酸脱氢酶来源于肠系膜明串珠菌(悬浮液)

CAS: 9001-40-5 货号: G128384 分子量: 55 kDa EC号: 232-602-6

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级别和纯度: 生物活性 ? ActiBioPure™ ? 天然 ? 高性能 ? EnzymoPure™ ? ≥360 NAD units/mg protein

Accession #

P11411

生物活性

≥360 NAD units/mg protein

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价格

数量

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¥1,499.90

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G128384-5KU

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¥4,499.90

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2-8°C储存。低温运输。请查阅批次 COA 获取详细规格。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 5 篇同行评审文献引用。

概述

The Leuconostoc GPDH exhibits dual coenzyme specificity, namely NAD and NADP. When assayed under conditions that are optimal for the particular coenzyme, the ratio of observed catalytic activity is NAD/NADP = 1.8.

Stability/Storage: The Leuconostoc mesenteroides glucose-6-phosphate dehydrogenase is a relatively stable enzyme in solution. The lyophilized and ammonium sulfate preparations are stable for 12 months when stored at 2-8°C.

Chromatographically purified. Phosphohexose isomerase, phosphogluconate dehydrogenase, adenylate kinase and creatine phosphokinase contaminant activities 0.011%, 0.002%, 0.0011% and 0.0011% respectively. A suspension in 3.7M ammonium sulfate.

Glucose-6-Phosphate Dehydrogenase Assay

Method

This dehydrogenase is rather unique in that it possesses dual coenzyme specificity. When assayed under conditions that are optimal for the particular coenzyme, the ratio of observed catalytic activity is NAD/NADP = 1.8. The reaction velocity is determined by measuring the increase in absorbance at 340 nm resulting from the reduction of NAD or NADP. One unit reduces one micromole of pyridine nucleotide per minute at 30°C and pH 7.8 under the specified conditions.

Reagents

0.055 M Tris⋅HCl buffer pH 7.8 containing 0.0033 M magnesium chloride

0.006 M Nicotinamide adenine dinucleotide phosphate, monosodium salt, (NADP), (TPN). Note: NADP may vary in salt form and degree of hydration. Care must be exercised to use an analytical grade and the correct molecular weight. When using NAD, prepare 0.06 M solution.

0.1 M Glucose-6-phosphate. As above, an analytical grade and the correct molecular weight must be used.

5 mM Glycine buffer, pH 8.0

5 mM Glycine buffer, pH 8.0 containing 0.1% bovine serum albumin

Enzyme

Dissolve lyophilized enzyme or dilute enzyme suspension to 1.0 mg/ml in 5 mM glycine buffer, pH 8.0. Care should be taken not to shake the solution as precipitation may result. Dilute further immediately before use in 5 mM glycine buffer, containing 0.1% albumin, to obtain a rate of 0.02 - 0.045 ΔA/min.

Procedure

Adjust spectrophotometer to 340 and 30°C.

Pipette into each cuvette as follows:

0.055 M Tris⋅HCl buffer, pH 7.8 with 0.0033 M MgCl2 2.7 ml

0.006 M NADP (or 0.06 M NAD) 0.1 ml

0.1 M Glucose-6-phosphate 0.1 ml

Incubate in spectrophotometer at 30deg.C for 7 - 8 minutes to achieve temperature equilibration and establish blank rate, if any. Add 0.1 ml diluted enzyme and record increase in A340/min for 4 to 5 minutes. Calculate A340/minute from the initial linear portion of the curve.

Calculation

规格

产品名称

葡萄糖-6-磷酸脱氢酶来源于肠系膜明串珠菌(悬浮液)

英文别名

G-6-P-DH | G6PD | G6PD | glucose-6-phosphate dehydrogenase (NADP(+)) | G6PDH | glucose-6-phosphate 1-dehydrogenase | glucose-6-phosphate dehydrogenase | GPD | zwf

规格或纯度

生物活性,ActiBioPure™,高性能,EnzymoPure™,天然,≥360 NAD units/mg protein

生化机理

催化葡萄糖6-磷酸氧化为6-磷酸葡萄糖酸内酯。可以利用NADP+或NAD+。

生物活性

≥360 NAD units/mg protein

种属

肠系膜明串珠菌

序列

Belongs to the glucose-6-phosphate dehydrogenase family.

Accession #

P11411

来源

天然

预测分子量

108.6 kDa

CAS编号和信息

9001-40-5

酶学委员会编号

1.1.1.49

分子类型

酶

储存与运输

物理形态

液体

储存缓冲液

A suspension in 3.7M ammonium sulfate.

浓度

≥360 NAD units/mg protein

储存条件

2-8°C储存

运输条件

低温运输

单位定义

One Unit reduces one micromole of NAD per minute at 30°C, pH 7.8, using glucose-6-phosphate as substrate.

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找到8个结果

批号(Lot Number)	证书类型	货号
B2422008	分析证书	G128384
H2217287	分析证书	G128384
D2624156	分析证书	G128384
D2624157	分析证书	G128384
L2525352	分析证书	G128384
L2525355	分析证书	G128384
H2512091	分析证书	G128384
H2217286	分析证书	G128384

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引用文献

1. Tengfei Xiong, Mingyue Yu, Jiang Zhu, Kai Tian, Mei Li, Xinghui Qiu. (2024) Functional characterization of Helicoverpa assulta CYP6B6 in insecticide metabolism. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, [PMID:38685236] [10.1016/j.pestbp.2024.105857]
2. Bohan Liang, Jiaojiao Ma, Lingmei Wang, Junxia Zhang, Yan Shang, Yanjie Li, Huazhen Zhu, Yunhua Teng, Shiqi Dong, Huirong Fan. (2025) Investigating the ‘RT-PK’ phenomenon: Effects of X-ray radiation on cabozantinib metabolism. EUROPEAN JOURNAL OF PHARMACOLOGY, [PMID:40541594] [10.1016/j.ejphar.2025.177862]
3. Yuhan Zhao, Xiaojie Zhou, Mei Li, Xinghui Qiu. (2025) Functional characterization of CYP9J26, a cytochrome P450 associated with insecticide resistance in the arbovirus vector Aedes albopictus. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:40533012] [10.1016/j.ijbiomac.2025.145280]
4. Yuhan Zhao, Xiaojie Zhou, Mei Li, Xinghui Qiu. (2025) Aedes albopictus CYP6Z8 metabolizes multiple AChE-inhibiting insecticides. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, [PMID:41629003] [10.1016/j.pestbp.2025.106934]
5. Zhuoda Liu, Yifei Zhou, Dongliang Wang, Ruichi Li, Zhen Tian, Yifan Li, Jiyuan Liu. (2026) From Field Overexpression to Biochemical Function: PxCYP6B4-Mediated Hydroxylation Confers Pyrethroid Resistance in Plutella xylostella. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, [PMID:41645610] [10.1021/acs.jafc.6c00843]

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葡萄糖-6-磷酸脱氢酶 来源于肠系膜明串珠菌(悬浮液)

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葡萄糖-6-磷酸脱氢酶来源于肠系膜明串珠菌(悬浮液)