计算溶液所需的质量、体积或浓度。
EnzymoPure™,生物活性,重组 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
Galacturonokinase (AtGalAK), EC 2.7.1.44, derived from Arabidopsis thaliana. It is applied in UDP‑GalA synthesis system, catalyzing the conversion of GalA to UDP‑GalA together with AtUSP.
Reaction Equation
ATP + GalA → GalA‑1‑P + ADP
Usage
Add substrates and 5 mM MgCl₂ to the reaction system. Add appropriate buffer salt such as Tris‑HCl to adjust pH to 7.5, then add a suitable amount of AtGalAK according to reaction requirements. After mixing, incubate the sample at 37 °C for 2‑4 h.
Terminate the reaction by boiling water bath for 5 min, then centrifuge at 10000 rpm for 10 min. Collect the supernatant for HPLC/TLC analysis.
Note
For scaled‑up reactions, incubation time or enzyme dosage should be adjusted according to practical conditions; low‑speed shaking mixing is acceptable.
Precautions
For your safety and health, wear a lab coat and disposable gloves during operation.
This product is for research use only!
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