亚基蓝（Methylene blue）又称亚甲蓝、美蓝、次甲基蓝、次甲蓝等，含水亚甲基蓝的分子式为C₁₆H₂₄ClN₃O₃S，分子量为373.9，CAS号为7220-79-3。甘油-亚甲蓝染色液由甘油、亚甲蓝、去离子水组成，可用于检查粪便的各种蠕虫卵。该试剂仅用于科研领域，不适用于临床诊断或其他用途。

自备仪器和试剂

不锈钢、塑料或纸平板
亲水性玻璃纸条

操作步骤（仅供参考）

1、准备玻璃纸条：将玻璃纸浸润于甘油-亚甲蓝染色液至少大于24h。

2、置少量粪便样本于报纸或小纸片上，用滤网在粪便标本上加压，使部分粪便样本通过滤网积聚于网上。

3、用刮片横刮滤网以收集筛过的粪便样本。

4、取带孔平板置于载玻片中央位置，用刮片时孔内填满粪便样本，并用刮片边缘横刮板面以去除孔边过多的粪便。

5、小心取下平板，使粪便样本呈矮小圆柱状留在玻片上。

6、玻璃纸条浸泡于甘油-亚甲蓝染色液，取出并覆盖于粪便上。

7、翻转玻片，在另一张玻片或在表面平滑、坚硬的物体上，朝向玻璃纸条挤压粪便样本，以使标本在玻片与玻璃纸条间均匀散开。澄清后，应能透过涂片读出本说明书上的字迹。

8、轻轻从侧面滑动并移下上层玻片，避免与玻璃纸条分离或使之掀起，将玻片置于实验台上，玻璃纸条面朝上。目的玻片（除钩虫卵外）应置于室温一至数小时，以使样本清晰。

9、以上下或横向移动方式检查涂片，并报告所发现的每种虫卵的计数，然后乘以适用的数值得出每克粪便中虫卵的数目。如使用20mg平板，乘以50；使用41.7mg平板，乘以24；使用50mg平板，乘以20。

注意事项

1、刮片和滤网经仔细清洗后，可重复使用。

2、粪便样本较干燥时，覆盖粪便的玻璃纸条必须湿润；如为软便，覆盖粪便的玻璃纸条的水分可略少。在干燥的气候条件下，过多的甘油只能延缓而不能防止粪便样本的干燥。

3、本法制片中的蛔虫及鞭虫卵可保存较长时间：血吸虫卵可保存数月：钩虫卵在制片30~60min后往往就观察不到了。

4、该染色法对粪膜厚度和透明时间有要求，如粪膜厚透明时间短，虫卵难以发现；如透明时间长则虫卵变型，不易辨认。如果检查钩虫卵，透明时间宜控制在30min以内。

5、为了您的安全与健康，请穿实验服并佩戴一次性手套操作。

6、本产品仅用于科研，严禁他用。

Methylene Blue (also known as methylthioninium chloride, methylene blue, basic blue 9, etc.), with the molecular formula of its hydrated form as C₁₆H₂₄ClN₃O₃S, a molecular weight of 373.9, and CAS number 7220-79-3. Glycerin–Methylene Blue Staining Solution is composed of glycerin, methylene blue, and deionized water, and can be used to examine various helminth eggs in feces. This reagent is for research use only and is not suitable for clinical diagnosis or other purposes.

Reagents, consumables and Equipments not provided

Stainless steel, plastic, or paper plate
Hydrophilic glass paper strips

Operating Steps (For Reference Only)

1. Prepare glass paper strips: Soak the glass paper in Glycerin–Methylene Blue Staining Solution for at least 24 hours.

2. Place a small amount of fecal sample on newspaper or a small piece of paper. Press a filter screen onto the fecal specimen so that a portion of the sample passes through and accumulates on the screen.

3. Use a spatula to scrape across the filter screen to collect the sieved fecal sample.

4. Place a perforated plate in the center of a glass slide. Fill the hole with fecal sample using the spatula, and use the edge of the spatula to scrape across the plate surface to remove excess sample around the hole.

5. Carefully remove the plate, leaving a short cylindrical fecal sample on the slide.

6. Take a glass paper strip soaked in the staining solution and cover the fecal sample with it.

7. Flip the slide and press the fecal sample against another slide or a smooth, hard surface to spread the specimen evenly between the slide and the glass paper strip. After clarification, the text on this instruction sheet should be readable through the smear.

8. Gently slide and remove the top slide from the side, avoiding separation from or lifting of the glass paper strip. Place the slide on the bench with the glass paper strip facing upward. For target slides (except for hookworm eggs), allow to stand at room temperature for one to several hours until the sample clarifies.

9.Examine the smear by moving vertically or horizontally. Report the count of each type of egg found, then multiply by the appropriate factor to obtain the number of eggs per gram of feces:

For a 20 mg plate, multiply by 50.
For a 41.7 mg plate, multiply by 24.
For a 50 mg plate, multiply by 20.

Precautions

The spatula and filter screen can be reused after careful cleaning.
For drier fecal samples, the covering glass paper strip must be well moistened. For soft stools, the glass paper strip may contain slightly less moisture. In dry climates, excess glycerin can only delay, not prevent, drying of the fecal sample.
In preparations made by this method:
- Ascaris and Trichuris eggs can be preserved for a relatively long time;
- Schistosome eggs can be preserved for several months;
- Hookworm eggs often become unobservable 30–60 minutes after preparation.
This staining method requires specific fecal film thickness and clarification time. If the film is too thick and clarification time is too short, eggs are difficult to detect. If clarification time is too long, eggs may deform and become hard to recognize. For examining hookworm eggs, the clarification time should be controlled within 30 minutes.
For your safety and health, please wear a lab coat and disposable gloves during operation.
This product is for research use only; any other use is strictly prohibited.

规格

英文别名

Glycerol-Methylene Blue Stain

规格或纯度

BioReagent,生物染色剂

稳定性与储存

Store at room temperature long term (12 months).

英文名称

Glycerol-Methylene Blue Staining Solution

储存条件

室温

运输条件

常规运输

产品线

生物产品

名称和识别符

EC号

615-731-6

分子类型

生物试剂/缓冲液

Isomeric SMILES

CN(C)C1=CC2=C(C=C1)N=C3C=CC(=[N+](C)C)C=C3S2.O.O.O.[Cl-]

分子量

373.9

Beilstein号

3599847

Reaxy-Rn

16692608

Reaxys-RN link address

https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=16692608&ln=

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高级数据

化学和物理性质

熔点

235°C

安全和危险性（GHS）

象形图

GHS08, GHS07

信号词

Warning

危险声明

H315: 引起皮肤刺激

H319: 引起严重眼睛刺激

H335: 可能引起呼吸道刺激

H373: 通过长时间或反复暴露对器官造成损害

H302: 吞食有害

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P305+P351+P338: 如进入眼睛：用水小心冲洗几分钟。如戴隐形眼镜并可方便地取出，取出隐形眼镜。继续冲洗。

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P302+P352: 如皮肤沾染：用水充分清洗。

P321: 特殊处理（请参阅此标签上的...）。

P405: 密闭存放

P501: 将内容物/容器处理到。。。

P264: 处理后要彻底洗手。

P260: 不要吸入灰尘/烟雾/气体/雾/蒸汽/喷雾。

P271: 仅在室外或通风良好的地方使用。

P270: 使用本产品时，请勿进食、饮水或吸烟。

P304+P340: 如误吸入：将人转移到空气新鲜处，保持呼吸舒适体位。

P403+P233: 存放在通风良好的地方。保持容器密闭。

P362+P364: 脱掉沾污的衣服，清洗后方可重新使用。

P330: 漱口

P264+P265: 处理后彻底洗手[和…]。不要触摸眼睛。

P301+P317: 如果被吞咽：请寻求医疗帮助。

P337+P317: 如果眼睛刺激持续：寻求医疗帮助。

P332+P317: 如果出现皮肤刺激：请寻求医疗帮助。

P319: 如果你感到不适，请寻求医疗帮助。

WGK Germany

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批号(Lot Number)	证书类型	货号
ZJ26F0332842	分析证书	G1510336

技术文档和文章

g1510336_甘油_亚甲蓝染色液产品说明书.pdf

Romanowsky染色体系的显色基础、方法类型与结果判读

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Romanowsky Staining Systems: Chromogenic Basis, Method Types, and Result Interpretation

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