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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β-1,3 葡聚糖酶(β-1,3-GA,EC 3.2.1.39)主要存在植物中,催化β-1,3-葡萄糖苷键水解。在植物染病或处于其他逆境条件下,可诱导细胞大量合成β-1,3-GA,因此β-1,3-GA 活性测定广泛应用于植物病理和逆境生理研究。 本试剂盒检测原理是β-1,3-GA 水解昆布多糖,内切β-1,3-葡萄糖苷键,产生还原末端,通过测定还原糖生成速率,来计算其酶活性。
β-1,3-Glucanase (β-1,3-GA, EC 3.2.1.39) is mainly found in plants and catalyzes the hydrolysis of β-1,3-glucosidic linkages. When plants are infected by pathogens or under other stress conditions, cells can be induced to synthesize large amounts of β-1,3-GA. Therefore, the assay of β-1,3-GA activity is widely used in studies of plant pathology and stress physiology. The detection principle of this kit is based on β-1,3-GA hydrolyzing laminarin, endohydrolyzing β-1,3-glucosidic linkages to produce reducing ends. The enzyme activity is calculated by measuring the rate of reducing sugar production.