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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
β-1,3-GA( EC 3.2.1.73)主要存在植物中，催化β-1,3-葡萄糖苷键水解。在植物染病或处于其他逆境条件下，可诱导细胞大量合成β-1,3-GA，因此β-1,3-GA活性测定广泛应用于植物病理和逆境生理研究。 测定原理：β-1,3-GA水解昆布多糖，内切β-1,3-葡萄糖苷键，产生还原末端，通过测定还原糖生成速率来计算其酶活性。
β-1,3-GA (EC 3.2.1.73) is mainly found in plants and catalyzes the hydrolysis of β-1,3-glucosidic bonds. Under conditions such as plant infection or other stresses, the synthesis of β-1,3-GA in cells is induced in large quantities. Therefore, the determination of β-1,3-GA activity is widely used in research on plant pathology and stress physiology. Determination Principle: β-1,3-GA hydrolyzes laminarin by cleaving endo-β-1,3-glucosidic bonds, producing reducing ends. The enzyme activity is calculated by measuring the rate of reducing sugar production.
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