计算溶液所需的质量、体积或浓度。
β-1,3-GA( EC 3.2.1.73)主要存在植物中,催化β-1,3-葡萄糖苷键水解。在植物染病或处于其他逆境条件下,可诱导细胞大量合成β-1,3-GA,因此β-1,3-GA活性测定广泛应用于植物病理和逆境生理研究。 测定原理:β-1,3-GA水解昆布多糖,内切β-1,3-葡萄糖苷键,产生还原末端,通过测定还原糖生成速率来计算其酶活性。
β-1,3-GA (EC 3.2.1.73) is mainly found in plants and catalyzes the hydrolysis of β-1,3-glucosidic bonds. Under conditions such as plant infection or other stresses, the synthesis of β-1,3-GA in cells is induced in large quantities. Therefore, the determination of β-1,3-GA activity is widely used in research on plant pathology and stress physiology. Determination Principle: β-1,3-GA hydrolyzes laminarin by cleaving endo-β-1,3-glucosidic bonds, producing reducing ends. The enzyme activity is calculated by measuring the rate of reducing sugar production.