来自杏仁的糖肽酶 A 用于去糖基化。它催化去除 N-连接的寡糖链并将 Asn 残基转化为 Asp。应用:
杏仁中发现的糖肽酶可分为 A、B 和 C 三组。糖肽酶 A 的最适 pH 值和等电点分别为 6.0 和 7.7。它偏爱长链糖肽。它还能够水解完整的糖蛋白,例如去唾液酸化的人转铁蛋白、卵清蛋白等。这些蛋白质在它们的 β-天冬氨酰-葡糖基胺键处切割具有去唾液酸碳水化合物部分的糖蛋白。
Glycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine linkages.
Application:
Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.