透明质酸酶来源于牛睾丸

CAS: 37326-33-3 货号: H128644 EC号: 253-464-3

20篇引用文献 COA

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级别和纯度: 生物活性 ? ActiBioPure™ ? 天然 ? 高性能 ? EnzymoPure™ ? ≥300 USP/NF units/mg dry weight

Accession #

Q5E985,Q8SQG8

生物活性

≥300 USP/NF units/mg dry weight

规格

库存

价格

数量

10KU

H128644-10KU

期货

¥363.90

50KU

H128644-50KU

现货

¥1,399.90

300KU

H128644-300KU

期货

¥5,399.90

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概述规格储存与运输技术文档高级数据三维结构质检证书（CoA,COO,BSE/TSE 和分析图谱) 此产品的引用文献技术文档和文章引用文献溶液计算器

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为什么选择此级别

ActiBioPure™,EnzymoPure™,天然,生物活性,高性能级，适用于对基线干扰要求严格的色谱和分析工作流程。

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储存与运输

-20°C储存。超低温运输。请查阅批次 COA 获取详细规格。

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质量文档

SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。

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文献证明

在色谱分析、有机合成和交叉偶联反应领域已被 20 篇同行评审文献引用。

概述

Testicular hyaluronidase is a glycoprotein containing 5% mannose and 2.7% glucosamine. Optimum pH range is 4.5-6.0. The enzyme catalyzes the hydrolysis of endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfate A and C (but not B), primarily to tetrasaccharide residues.

Hyaluronidase catalyzes the random hydrolysis of 1,4-linkages between 2-acetamido-2-deoxy-b-D-glucose and D-glucose residues in hyaluronate.

Characteristics of Hyaluronidase from Bovine Testes:

Specificity

Testicular hyaluronidase hydrolyzes the endo-N-acetylhexosaminic bonds of hyaluronic acid and chondroitin sulfuric acids A and C (but not B), primarily to tetrasaccharide residues. Monosaccharides are not liberated.

Composition

The enzyme is a glycoprotein containing 5% mannose and 2.17% glucosamine. The amino acid composition has been determined.

Characteristics of Hyaluronidase

Molecular Weight: Four subunits of 14,000 each and a total molecular weight of 55,000.

Optimal pH: 4.5 - 6.

Extinction Coefficient: extinction coefficient= approximately 8.

Inhibitors: Fe2+ and Fe3+ are inhibitory as are Mn2+ and Cu2+.

Stability: Hyaluronidase is stable for 1 - 2 years and the purified preparation for 6 - 12 months when stored at -20°C.

Stabilizers: Sodium chloride

Hyaluronidase Assay

Method

Hyaluronic acid is measured by its ability to form turbidity with an acid albumin solution. Turbidity is a function of hyaluronic acid concentration and can hence be related to enzyme activity. One unit is based on the change in absorbency (turbidity) at 540nm of an internal standard assayed concurrently with each lot. Internal standard replaces USP/NF reference no longer available.

Reagents

1. 0.1 M Sodium phosphate buffer, pH 5.3 with 0.15 M sodium chloride (HSE buffer)

2. 0.5 M Sodium acetate buffer, pH 4.2

3. Albumin reagent: Prepare by dissolving 2.5 grams of bovine serum albumin, Fraction V in 250 ml of 0.5 M sodium acetate buffer, pH 4.2. Adjust pH to 3.0 with 2 N HCl and heat at 93°C for 30 minutes. Cool and adjust final volume to 1000 ml with 0.5 ml sodium acetate buffer, pH 4.2.

4. Standard: Prepare stock solutions of 1.0 and 0.5 mg/ml.

5. Hyaluronic acid (HA): Dissolve 10 mg Worthington hyaluronic acid in 25 ml 0.1 M sodium phosphate buffer: pH 5.3 with 0.15 M sodium chloride. Note: This solution can be prepared by allowing VHHA to dissolve overnight. Heating in a boiling water bath for 10 - 15 minutes is the preferred method if the material is not immediately soluble.

Enzyme

Prepare stock solution of enzyme at one mg/ml in 0.1 M sodium phosphate buffer pH 5.3 with 0.15 M sodium chloride. Immediately prior to use dilute further in the same buffer. For crude grade material concentrations of 0.01 - 0.05 mg/ml are recommended. For purified grade concentration of 0.001 - 0.01 mg/ml are recommended.

Procedure:

I. Standard Curve

Into a series of numbered tubes, pipette as follows:

Place all tubes in a boiling water bath for 5 minutes. Cool to room temperature. Add 9.0 ml of albumin reagent and allow to stand for 10 minutes. Read absorbance at 540 nm. Plot absorbance at 540 nm versus mg HA to form standard curve.

Hyaluronic acid should be soluble under the defined conditions and should produce a standard curve with a slope of 1.5 or greater.

II. Test Procedure

Pipette 0.5 ml of a 0.4 mg/ml hyaluronic acid solution into a series of test tubes. Incubate at 37°C for 4 - 5 minutes to achieve temperature equilibrium. Incubate one blank tube with one ml of 0.1 M sodium phosphate buffer, pH 5.3 with 0.15 M sodium chloride. At timed intervals add 0.5 ml of appropriately diluted enzyme or standard to respective tubes. Incubate each tube exactly 10 minutes and cool in an ice bath to room temperature. Add 9.0 ml of albumin reagent to each tube and incubate at room temperature for 10 minutes. Read A540 of each tube versus the blank.

Calculation

Determine the amount of hyaluronic acid remaining after digestion from the standard curve.

Calculate the amount of hyaluronic acid digested as follows:

mg HA digested = 0.2 mg - mg HA remaining

规格

产品名称

透明质酸酶来源于牛睾丸

别名

玻璃酸酶 | 玻璃糖醛酸酶

英文别名

规格或纯度

生物活性, ActiBioPure™, 天然, 高性能, EnzymoPure™, ≥300 USP/NF units/mg dry weight

生化机理

透明质酸酶降解透明质酸，并被发现在癌症进展过程中受到不适当的调节。这些酶随机切割透明质酸，软骨素和硫酸软骨素中的β-N-乙酰己糖胺-[1→4]糖苷键。

生物活性

≥300 USP/NF units/mg dry weight

种属

牛(Bovine)

序列

Belongs to the glycosyl hydrolase 56 family.

Accession #

Q5E985,Q8SQG8

来源

天然

预测分子量

~55 kDa

作用类型

激活剂

CAS编号和信息

37326-33-3

酶学委员会编号

3.2.1.35

分子类型

酶

储存与运输

物理形态

冻干(Lyophilized)

浓度

≥300 USP/NF units/mg dry weight

复溶

Reconstitute in 0.1M sodium phosphate buffer, pH 5.3 with 0.15M sodium chloride.

储存条件

-20°C储存

运输条件

超低温运输

单位定义

One unit is based on the change in absorbency (turbidity) at 540nm of an internal standard assayed concurrently with each lot. Internal standard replaces USP/NF reference no longer available.

技术文档

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找到6个结果

批号(Lot Number)	证书类型	货号
L2215645	分析证书	H128644
L2501379	分析证书	H128644
L2515641	分析证书	H128644
L2410429	分析证书	H128644
D2402023	分析证书	H128644
L2215647	分析证书	H128644

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