胃幽门螺杆菌 (Helicobacter Pyloric, HP) 又称胃幽门弯曲菌 (Campylobacter Pyloric)，现已证实这种细菌与慢性胃炎和消化性溃疡有密切关系，胃幽门螺杆菌一般呈弧形、S形或海鸥状，有时可见3～4个弯曲呈螺旋状，常呈鱼群状分布。该菌多见于胃黏膜表面上皮与黏膜层之间，并贴近表面上皮细胞，部分进入上皮细胞胞质内，胃小凹和黏膜浅层腺腔内亦有此菌。幽门螺旋杆菌染色主要有亚甲蓝法、硝酸银法、迈格林华-姬姆萨法(May-Grunwald-Giemsa，MGG法)、碱性品红法等，硝酸银法对比清楚，染片可以长期保存，但操作较为麻烦、耗时。其他方法较为简便，但染片容易褪色。
阿拉丁May-Grunwald-Giemsa 染色液主要由迈格林华染液和姬姆萨染液组成，染色后胃幽门螺旋杆菌呈蓝色，胶原纤维呈红色，红细胞呈绿色，胃黏膜上皮呈淡蓝色，细胞核呈深蓝色，HP多位于胃黏膜上皮表面的黏液中，特别在胃小凹中数量较多。该试剂仅用于科研领域，不宜用于临床诊断或其他用途。
产品组分及储存条件：
H1510399
| Component
| 2×100mL
| Storage
|
H1510399A
| May-Grunwald Stain
| 50mL
| RT. Store in the dark.
|
H1510399B
| MG 磷酸盐缓冲液
| 50mL
| RT
|
H1510399C
| Giemsa Stain
| 50mL
| RT. Store in the dark.
|
H1510399D
| Giemsa 磷酸盐缓冲液
| 50mL
| RT
|
取 A、B等量混合，即为 May-Grunwald 工作液，不宜预先配制。
取C、D等量混合，即为Giemsa工作液，不宜预先配制。
自备材料：
10%福尔马林固定液、蒸馏水、系列乙醇
操作步骤(仅供参考)：
1、组织固定于10%福尔马林，常规脱水包埋。
2、切片厚4μm，常规二甲苯或脱蜡透明液脱蜡至水。
3、再用蒸馏水洗1次。
4、把切片周围水分抹干，滴入May-Grunwald工作液数滴染色10min，倾去染液。
5、Giemsa工作液滴染20min，倾去染液。
6、无水乙醇快速洗去余液，稍烤干。
7、二甲苯或脱蜡透明液透明，中性树胶封固。
染色结果：
胃幽门螺旋杆菌
| 蓝色
|
胶原纤维
| 红色
|
红细胞
| 绿色
|
胃黏膜上皮
| 淡蓝色
|
细胞核
| 深蓝色
|
注意事项：
1、幽门螺旋杆菌染色液 (MGG法) 中 May-Grunwald 工作液和 Giemsa 工作液须于临用前配置，不宜保存。
2、最后的无水乙醇要速洗，否则容易脱色。
3、胃幽门螺旋杆菌仅用Giemsa工作液染色也可以显示出来，也较清晰。
4、为了您的安全和健康，请穿实验服并戴一次性手套操作。
5、试剂开封后请尽快使用，以防影响后续实验效果。
Helicobacter pylori (HP), also known as Campylobacter pyloric, has been proven to be closely associated with chronic gastritis and peptic ulcers. H. pylori is generally arc-shaped, S-shaped or seagull-like; sometimes it appears as a spiral with 3–4 curves, and is often distributed in a fish-school pattern. The bacteria are mostly found between the surface epithelium and the mucosal layer of the gastric mucosa, adhering closely to the surface epithelial cells, with some invading the cytoplasm of epithelial cells. They can also be detected in the gastric pits and the glandular lumens of the superficial mucosa. Common staining methods for H. pylori include the methylene blue method, silver nitrate method, May-Grunwald-Giemsa (MGG) method, and basic fuchsin method. The silver nitrate method yields clear contrast and the stained slides can be preserved for a long time, but the operation is relatively cumbersome and time-consuming. Other methods are more convenient, yet the stained slides are prone to fading.
Aladdin May-Grunwald-Giemsa (MGG) Staining Solution is mainly composed of May-Grunwald stain and Giemsa stain. After staining, H. pylori appears blue, collagen fibers are stained red, red blood cells show green, gastric mucosal epithelium turns pale blue, and cell nuclei are dark blue. HP is mostly located in the mucus on the surface of the gastric mucosal epithelium, and is particularly abundant in the gastric pits. This reagent is for research use only. It is not suitable for clinical diagnosis or any other purposes.
Product Components and Storage Conditions:
H1510399
| Component
| 2×100mL
| Storage
|
H1510399A
| May-Grunwald Stain
| 50mL
| RT. Store in the dark.
|
H1510399B
| MG Phosphate Buffer
| 50mL
| RT
|
H1510399C
| Giemsa Stain
| 50mL
| RT. Store in the dark.
|
H1510399D
| Giemsa Phosphate Buffer
| 50mL
| RT
|
Mix equal volumes of A and B to prepare the May-Grunwald working solution. Do not prepare in advance.
Mix equal volumes of C and D to prepare the Giemsa working solution. Do not prepare in advance.
Materials to Be Prepared by the User:
10% formalin fixative, distilled water, graded ethanol solutions
Operating Procedures (For Reference Only):
1. Fix the tissue in 10% formalin, then perform routine dehydration and embedding.
2. Cut sections with a thickness of 4 μm, and deparaffinize them to water using routine xylene or deparaffinizing clearing solution.
3. Rinse once more with distilled water.
4. Wipe dry the water around the sections, add several drops of May-Grunwald working solution and stain for 10 minutes, then pour off the staining solution.
5. Add Giemsa working solution dropwise and stain for 20 minutes, then pour off the staining solution.
6. Quickly rinse off the residual solution with anhydrous ethanol, and bake slightly to dry.
7. Clear the sections with xylene or deparaffinizing clearing solution, and mount with neutral balsam.
Staining Results:
Helicobacter pylori
| Blue
|
Collagen Fibers
| Red
|
Red Blood Cells
| Green
|
Gastric Mucosal Epithelium
| Pale Blue
|
Cell Nuclei
| Dark Blue
|
Precautions:
1. The May-Grunwald working solution and Giemsa working solution in the Helicobacter pylori Staining Solution (MGG Method) must be prepared immediately before use and are not suitable for storage.
2. The final rinse with anhydrous ethanol should be performed quickly; otherwise, the stain is liable to fade.
3. Helicobacter pylori can also be clearly visualized by staining with Giemsa working solution alone.
4. For your safety and health, wear a lab coat and disposable gloves during operation.
5. Please use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
ZJ26F0433800
危险品化学品经营许可证（带存储）