计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
主要用于人外周血淋巴细胞的分离。
全过程样本、试剂及实验环境均需在20±2℃的条件下进行（试剂需要复温，夏季20℃，冬季23℃）。
【注意事项】
1. 关于血液分离液效果讨论。
A. 脐带血：脐带血收集到细胞分离时长一般大于2小时，冷藏存放与运输在所难免。
B. 案例：应用过免疫动员剂类药物，普通的样本密度分离液分离效果较差，有大量的粒细胞混杂，不能使用。
2. 本实验最好不使用高聚合材质（如聚苯乙烯）的塑料制品，应使用无静电、低静电离心管及未经碱处理过后的玻璃制品，因为静电作用将导致细胞贴壁、碱处理的玻璃表面会变成毛面，影响细胞分离效果。
3. 吸取过多的淋巴细胞层及分离液层会导致分离液交界处的粒细胞被吸出从而使混杂的粒细胞数量增加。
4. 吸取过多的淋巴细胞层上层溶液会导致血浆蛋白及血小板混杂。
5. 不当的稀释方法会降低细胞得率及活性。稀释液要求：不含钙镁离子的缓冲液或培养基。血液样本经过稀释则分离过程中需适当降低离心力和离心时间。
It is mainly used for the isolation of human peripheral blood lymphocytes
The entire process, including samples, reagents and experimental environment, must be carried out under the condition of 20±2℃ (reagents need to be rewarmed to 20℃ in summer and 23℃ in winter).
Matters needing attention：
1.Discussion on the effect of blood separation medium
A. Umbilical cord blood: The interval between umbilical cord blood collection and cell separation is generally more than 2 hours, and refrigerated storage and transportation are inevitable.
B. Special case: For samples from subjects who have used immunomobilizer drugs, ordinary density separation medium has poor separation effect with a large amount of granulocyte contamination, and thus is not applicable.
2.It is not recommended to use high-polymer plastic products (such as polystyrene) in this experiment. Non-electrostatic/low-electrostatic centrifuge tubes and un-alkali-treated glass products should be used instead. Electrostatic interaction will cause cells to adhere to the wall, and alkali treatment will turn the glass surface into a rough surface, both of which will affect the cell separation effect.
3.Suctioning too much lymphocyte layer and separation medium layer will lead to sucking out granulocytes at the junction of the separation medium, thus increasing the number of contaminated granulocytes in the product.
4.Suctioning too much supernatant above the lymphocyte layer will lead to contamination of plasma protein and platelets.
5.Improper dilution method will reduce cell yield and viability. Dilution requirement: use buffer or medium free of calcium and magnesium ions. If the blood sample is diluted, the centrifugal force and centrifugation time need to be appropriately reduced during the separation process.
规格
产品名称
人外周血淋巴细胞分离液(FICOLL配制)
分子类型
未知
储存与运输
物理形态
液体
储存条件
避光,室温
运输条件
常规运输
技术文档
高级数据
质检证书（CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
输入批号以搜索分析图谱:
技术文档和文章
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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