计算溶液所需的质量、体积或浓度。
ActiBioPure™,EnzymoPure™,天然,生物活性,高性能 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
2-8°C储存。低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 1 篇同行评审文献引用。
Pure Isoamylase (Glycogen 6-glucanohydrolase) for use in biochemical enzyme assays and analytical testing applications. Isoamylase, Fructanase and Amyloglucosidase are used in the enzyme hydrolysis step of two validated methods for the determination of polydextrose (a low molar mass dietary fiber) in foods: AOAC method 2000.11 and Chinese GB Standard 5009.245-2016.
PROPERTIES
1. ELECTROPHORETIC PURITY:
- Single band on SDS-gel electrophoresis (MW = 71,500).
- Single major band on isoelectric focusing (pI = 5.0).
2. SPECIFICITY:
Hydrolysis of (1,6)-α-D-glucosidic branch linkages in glycogen, amylopectin and their β-limit dextrins.
3. PHYSICOCHEMICAL PROPERTIES:
Recommended conditions of use are at pH 4.0 and up to 40°C.
pH Optima: 4.0-5.0
pH Stability: 3.5-6.0 (16 h, 4°C)
Temperature Optima: 50°C
Temperature Stability: <45°C (pH 4.0, 15 min)
4. RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES:
Recommended for use in AOAC 2000.11 (polydextrose in food)
5. PRODUCT DETAILS:
The enzyme is supplied as an ammonium sulphate suspension containing 0.02% sodium azide and should be stored at 4°C. This enzyme is very unstable to freezing and thawing. DO NOT FREEZE. It is recommended that all buffers used for dilution contain BSA (1 mg/mL). Swirl to mix the enzyme immediately prior to use.
6. EXPERIMENTAL DATA:

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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | I1438585 | |
| 分析证书 | I1438585 |
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