计算溶液所需的质量、体积或浓度。
为什么选择此级别
，适用于对基线干扰要求严格的色谱和分析工作流程。
储存与运输
避光,室温。常规运输 。请查阅批次 COA 获取详细规格。
质量文档
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
文献证明
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
概述
苏木素 (Hematoxylin)和伊红 (Eosin)联合染色简称HE染色，是病理学和组织学最常用的一种染色方法。苏木精为碱性天然染料，可使细胞核着色，细胞核内染色质的主要成分是DNA，在DNA的双螺旋结构中，两条核苷酸链上的磷酸基向外，使DNA双螺旋的外侧带负电荷，呈酸性，很容易与带正电荷的苏木精碱性染料以离子键或氢键结合而被染色。
改良MacConaill铅苏木素染色液以铅盐作为氧化剂，可显示神经内分泌细胞。该试剂仅用于科研领域，不适用于临床诊断或其他用途。
| I1508462 | Component | 140 mL | Storage |
| I1508462A | MacConaill Differentiation | 100 mL | RT |
| I1508462B | MacConaill 铅溶液 | 10 mL | RT. Store in the dark |
| I1508462C | MacConaill 氧化剂 | 10 mL | RT |
| I1508462D | MacConaill 增强剂 | 1 mL | RT. Store in the dark |
| I1508462E | 苏木素染色液 | 20 mL | RT. Store in the dark |
临用前，按B:C:D=10:10:1 充分混匀即为MacConaill染色液，即配即用。
临用前，按MacConaill染色液 (BCD): 试剂 (E)=1:1 充分混匀，静置 30min 后过滤，每 3ml 滤液溶于15ml 蒸馏水，即为MacConaill苏木素染色液，即配即用。
操作步骤(仅供参考)：
1、切片脱蜡至水
①二甲苯或脱蜡透明液作用2次，每次5-10min。
②(可选)无水乙醇作用2次，每次3-5min。
③95%的乙醇: 3-5min
④90%的乙醇: 3-5min
⑤80%的乙醇: 3-5min
⑥自来水或蒸馏水冲洗: 1-3min
2、染色
①MacConaill Differentiation分化数秒。若用福尔马林、多聚甲醛、Bouin液固定组织，60℃分化3-4h；若用戊二醛、Helly液固定组织60-65℃分化12h。
②蒸馏水冲洗: 5-10s
③取配制好的MacConaill苏木素染色液提前预热至37-45℃，37℃恒温浸染2-3h或45℃恒温浸染1-2h。
④蒸馏水冲洗: 5-10min
3、脱水、透明、封固
①80%乙醇: 10-20s
②90%乙醇: 10-20s
③95%乙醇作用2次，每次1-2min。
④无水乙醇作用2次，每次2-3min。
⑤二甲苯透明3次，每次2-3min。
⑥中性树脂封片。
染色结果：
内分泌细胞颗粒: 深蓝色至黑色
肌肉、神经或其他组织: 蓝色至黑色
注意事项：
1、如果组织是用多聚甲醛或Helly液固定，染色时间应适当延长。
2、切片脱蜡应尽量干净。
3、系列乙醇应经常更换新液。
4、冷冻切片染色时间尽量要短。
5、为了您的安全和健康，请穿实验服并戴一次性手套操作。
6、试剂开封后请尽快使用，以防影响后续实验效果。
Combined staining with Hematoxylin and Eosin, abbreviated as HE staining, is the most widely used staining method in pathology and histology. Hematoxylin is a natural basic dye that stains cell nuclei. The main component of chromatin within the nucleus is DNA. In the DNA double helix structure, the phosphate groups on the two nucleotide chains face outward, rendering the outer surface of the DNA double helix negatively charged and acidic. This allows it to readily bind to the positively charged basic dye hematoxylin via ionic or hydrogen bonds, resulting in nuclear staining.
Modified MacConaill Lead Hematoxylin Stain Solution uses lead salts as oxidizing agents and can be used to visualize neuroendocrine cells. This reagent is for research use only and not intended for clinical diagnosis or other applications.
| I1508462 | Component | 140 mL | Storage |
| I1508462A | MacConaill Differentiation | 100 mL | RT |
| I1508462B | MacConaill Lead Solution | 10 mL | RT. Store in the dark |
| I1508462C | MacConaill Oxidizing Agent | 10 mL | RT |
| I1508462D | MacConaill Enhancer | 1 mL | RT. Store in the dark |
| I1508462E | Hematoxylin Staining Solution | 20 mL | RT. Store in the dark |
MacConaill Stain Solution: Mix Solution B, Solution C, and Solution D at a ratio of 10:10:1 immediately before use. Prepare and use the solution on-site.
MacConaill Hematoxylin Stain Solution: Mix the prepared MacConaill Stain Solution (BCD mixture) and Reagent E at a ratio of 1:1 thoroughly. Let the mixture stand for 30 minutes and then filter it. Dissolve 3 mL of the filtrate in 15 mL of distilled water. Prepare and use the solution on-site.
Operating Steps (For Reference Only)
1. Deparaffinization of Sections to Water
① Treat sections with xylene or deparaffinization clearing solution twice, 5-10 minutes each time.
② (Optional) Treat with anhydrous ethanol twice, 3-5 minutes each time.
③ 95% ethanol: 3-5 minutes.
④ 90% ethanol: 3-5 minutes.
⑤ 80% ethanol: 3-5 minutes.
⑥ Rinse with tap water or distilled water: 1-3 minutes.
2. Staining
① Differentiate with MacConaill Differentiating Solution for several seconds. For tissues fixed with formalin, paraformaldehyde, or Bouin's solution, differentiate at 60℃ for 3-4 hours; for tissues fixed with glutaraldehyde or Helly's solution, differentiate at 60-65℃ for 12 hours.
② Rinse with distilled water: 5-10 seconds.
③ Pre-warm the prepared MacConaill Hematoxylin Stain Solution to 37-45℃ in advance. Immerse sections in the solution at a constant temperature of 37℃ for 2-3 hours or at 45℃ for 1-2 hours.
④ Rinse with distilled water: 5-10 minutes.
3. Dehydration, Clearing, and Mounting
① 80% ethanol: 10-20 seconds.
② 90% ethanol: 10-20 seconds.
③ Treat with 95% ethanol twice, 1-2 minutes each time.
④ Treat with anhydrous ethanol twice, 2-3 minutes each time.
⑤ Clear with xylene three times, 2-3 minutes each time.
⑥ Mount with neutral resin.
Staining Results
Endocrine cell granules: Dark blue to black
Muscle, nerve, or other tissues: Blue to black
Precautions
1. For tissues fixed with paraformaldehyde or Helly's solution, appropriately extend the staining time.
2. Ensure thorough deparaffinization of sections.
3. Replace the graded ethanol series with fresh solutions regularly.
4. Minimize staining time for frozen sections.
5. For your safety and health, wear a lab coat and disposable gloves during operation.
6. Use the reagent as soon as possible after opening to avoid affecting subsequent experimental results.
规格
稳定性与储存
各组分在相应的储存条件下保质期1年。
英文名称
Modified MacConaill Lead Hematoxylin Staining Solution
储存条件
避光,室温
运输条件
常规运输
产品线
生物产品
名称和识别符
分子类型
生物试剂/缓冲液
技术文档
高级数据
化学和物理性质
敏感性
Light-sensitive
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 ZJ26F0333268 |
分析证书 | I1508462 |
溶液计算器
常见问题
本产品应如何储存？
请于室温、避光条件下保存。本品对光敏感，请保存在原始的避光或棕色容器中。
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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