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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
α-酮戊二酸脱氢酶（α-ketoglutarate dehydrogenase, α-KGDH）（EC 1.2.4.2）广泛存在于动物、植物、微生物和体外培养细胞的线粒体中，是三羧酸循环调控关键酶之一，可以催化α-酮戊二酸氧化脱羧生成琥珀酰辅酶A。 本试剂盒检测原理是α-KGDH 可以催化α-酮戊二酸、NAD+和辅酶 A 生成琥珀酰辅酶 A、二氧化碳和 NADH，NADH 在 340 nm 处有特征吸收峰，根据 NADH 的生成速率可计算获得α-KGDH 的活性。
α-Ketoglutarate dehydrogenase (α-KGDH) (EC 1.2.4.2) is widely present in mitochondria of animals, plants, microorganisms, and cultured cells in vitro. It is one of the key regulatory enzymes in the tricarboxylic acid cycle, catalyzing the oxidative decarboxylation of α-ketoglutarate to form succinyl-CoA. The assay principle of this kit is based on the reaction in which α-KGDH catalyzes α-ketoglutarate, NAD⁺, and coenzyme A to generate succinyl-CoA, carbon dioxide, and NADH. NADH exhibits a characteristic absorption peak at 340 nm. The activity of α-KGDH can be calculated by measuring the rate of NADH generation.
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