计算溶液所需的质量、体积或浓度。
BioReagent,无菌,生物染色剂,用于显微镜,适用于免疫组织化学(for IHC),适用于免疫荧光(IF),适用于荧光分析 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
避光,-20°C储存。超低温运输 。请查阅批次 COA 获取详细规格。
SDS、COA、产品数据表及规格说明书均可下载。可通过批号查询获取批次 COA。
在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
LS 510 Free Acid是一款高性能荧光标记染料,核心优势在于其独特的长斯托克斯位移 (Stokes shift)光学特性,激发波长为509 nm、发射波长为590 nm,显著的波长差可有效减少激发光与发射光的光谱重叠,从根本上降低信号串扰干扰。该产品设计目的是解决传统荧光染料在多路复用实验中因光谱重叠导致的检测准确性不足问题,其不仅具备优异的溶解性,更凭借长斯托克斯位移实现多通道标记时的信号区分度提升,同时在荧光稳定性与生物相容性上表现均衡,能满足精准标记的基础需求。
LS 510 Free Acid作为免疫荧光实验中常用工具,可广泛应用于细胞骨架动态观测、免疫组化、荧光原位杂交等多标记实验场景,尤其适用于需要同时检测多种靶点的复杂样本分析,其长斯托克斯位移特性,有效避免了在多重检测过程中由于信号通道间的串扰而导致的信号重叠问题,同时避免信号干扰,提升多靶点检测的准确性。
注:效能等同于Dyomics DY-510XL。
应用范围:细胞骨架动态观测;免疫组化;荧光原位杂交;多靶点复杂样本分析;免疫荧光等;
产品特点:
1、高亮度荧光:强效的发射能力,亮度超越FITC,具有更高的灵敏度;
2、卓越的光稳定性:对光漂白具有极强的抵抗力,确保定量分析数据和结果的稳定性;
3、缓冲液稳定性:偶联的荧光团在pH 4至pH 9的范围内保持完全稳定;
4、低荧光淬灭和通道串扰:Ex/Em:509/590 nm,长斯托克斯位移,Ex-Em间隔达81 nm;
5、仪器兼容性:激发和发射光谱与所有主流荧光检测设备的滤光片组和激光设置相匹配。
产品参数:
1、Ex/Em:509/590 nm;
2、Extinction Coefficient (ε):50000;
3、Molecular Weight:605.14;
4、溶解性:可溶于水、DMSO、DMF;
5、光谱图:

注意事项:
1、本产品可溶于DMSO、DMF、水,使用不同溶剂,激发和发射波长可能会发生变化。
2、使用前请充分摇匀,避免反复冻融。建议首次使用时将产品分装至小管中,并储存在-20 ℃。
3、荧光染料易受光照影响而淬灭,请务必避光操作,以保持染料稳定性。
4、本产品仅限于科研用途并且不得存放于普通住宅内。
5、为了您的安全和健康,请遵循您所在常规实验室安全规定。
LS 510 Free Acid is a high-performance fluorescent labeling dye featuring a unique long Stokes shift. With an excitation wavelength of 509 nm and an emission wavelength of 590 nm, its large spectral separation effectively reduces overlap between excitation and emission light, thus minimizing spectral crosstalk at the source. This product is designed to address the insufficient detection accuracy caused by spectral overlap of conventional fluorescent dyes in multiplex experiments. It offers excellent solubility and enhanced signal discrimination in multi-channel labeling due to its long Stokes shift. It also provides balanced performance in fluorescence stability and biocompatibility, meeting the core requirements for precise labeling.
As a widely used reagent in immunofluorescence assays, LS 510 Free Acid is suitable for cytoskeleton dynamics imaging, immunohistochemistry, fluorescence in situ hybridization, and other multi-labeling applications. It is especially ideal for the analysis of complex samples requiring simultaneous detection of multiple targets. Its long Stokes shift effectively eliminates signal overlap and interference between detection channels, improving the accuracy of multi-target detection.
Note: Performance is equivalent to Dyomics DY‑510XL.
Applications
Cytoskeleton dynamics imaging; Immunohistochemistry; Fluorescence in situ hybridization (FISH); Multi-target complex sample analysis; Immunofluorescence
Product Features
1. High brightness: Strong emission intensity, brighter than FITC, with higher sensitivity.
2. Excellent photostability: High resistance to photobleaching, ensuring reliable quantitative data and stable results.
3. Buffer stability: The conjugated fluorophore remains fully stable in the pH range of 4 to 9.
4. Low photobleaching and channel crosstalk: Ex/Em: 509/590 nm; long Stokes shift with an Ex‑Em gap of 81 nm.
5. Instrument compatibility: Excitation and emission spectra match filter sets and laser configurations of all major fluorescence detection instruments.
Product Parameters
1. Ex/Em: 509/590 nm
2. Extinction Coefficient (ε): 50000
3. Molecular Weight: 605.14
4. Solubility: Soluble in water, DMSO, DMF
5. Spectrum:

Precautions
1. This product is soluble in DMSO, DMF, and water. Excitation and emission wavelengths may vary slightly in different solvents.
2. Mix thoroughly before use and avoid repeated freeze‑thaw cycles. Upon first use, aliquot the product into small tubes and store at ‑20 °C.
3. Fluorescent dyes are prone to photobleaching; always protect from light during handling to maintain stability.
4. This product is for research use only and must not be stored in residential areas.
5. For your safety and health, please follow the standard laboratory safety regulations of your institution.
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
| 分析证书 | L1511496 |