计算溶液所需的质量、体积或浓度。
BioReagent,EnzymoPure™,for DNA and RNA applications,for Southern blotting,PCR试剂,分子生物学级,用于DNA合成,用于体外转录 级 ,适用于对基线干扰要求严格的色谱和分析工作流程。
-20°C储存,避免反复冻融。超低温运输 。请查阅批次 COA 获取详细规格。
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在色谱分析、有机合成和交叉偶联反应领域已被 0 篇同行评审文献引用。
SalI 是一种 Type IIP 类的限制性内切酶,可识别 G^TCGAC 回文序列,适用于酶切质粒、PCR 产物等,可在 15-30 min 内完成 DNA 切割反应。SalI 和 10×Cut Reaction Buffer 均添加了重组白蛋白(rAlbumin),确保了产品的安全性及稳定性。
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来源
E.coli
酶活定义
在 50 μl 反应体系中,37℃下,1小时内酶切1 μg λDNA (HindIII 酶切)所需的酶量定义为 1 个活力单位(U)。
酶切位点
5’…G↓TCGAC…3’
3’…CAGCT↑G…5’
产品使用步骤
(1)配置反应体系,如下表所示
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(2)混匀,于37℃孵育15-30min。
(3)65°C孵育 20 min,终止反应。
注:*Sal 最后加入体系中,一般50u 反应体系加入1 μl的Sall,也可根据实验进行调整,建议酶的加入量不超过总体系 的 10%,避免因甘油含量过高产生星活性。
注意事项
(1)Sall 对 Dam、Dcm 甲基化不敏感;对 CpG 甲基化敏感。
(2)在酶切反应前,尽量确保 DNA 不含苯酚、氯仿、酒 精、EDTA等杂质,以免影响酶切效果。
(3)本产品仅作科学研究使用,不得用于其它用途。
SalI is a Type IIP restriction enzyme, which can recognize G^TCGAC palindromic sequence, and is suitable for enzyme cleavage plasmid, PCR products, etc., and can complete DNA cleavage reaction within 15-30 min. Recombinant albumin was added to both SalI and 10×Cut Reaction Buffer to ensure the safety and stability of the product.
Source
E.coli
Enzyme Activity Definition
In a 50 μl reaction system, the amount of enzyme required for enzymatic digestion of 1 μg λDNA (HindIII enzyme digestion) within 1 hour at 37℃ is defined as 1 unit of activity (U).
Restriction Enzyme CuttingSite
5’…G↓TCGAC…3’
3’…CAGCT↑G…5’
Protocol
(1) Configure the reaction system, as shown in the following table
(3) Incubation at 65°C for 20 min terminated the reaction. Note :*Sal is finally added to the system, the general 50u reaction system adds 1μl of Sall, and can also be adjusted according to the experiment, it is recommended that the amount of enzyme added does not exceed 10% of the total system to avoid star activity due to high glycerol content. Matters needing attention (1)Sall is not sensitive to Dam and Dcm methylation; It is sensitive to CpG methylation. (2) Before the enzyme digestion reaction, try to ensure that the DNA does not contain impurities such as phenol, chloroform, alcohol, EDTA, so as not to affect the effect of enzyme digestion. (3) This product is for scientific research only and shall not be used for other purposes. |