计算溶液所需的质量、体积或浓度。
有货
概述
分子量：mol wt 120 kDa (two subunits)
Product introduction
Firefly luciferase is an enzyme that catalyzes production of light from luciferin in the presence of Mg2+-ATP and oxygen. The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase activity can be inhibited by general anesthetics including isoflurane and ketamine/medetomidine thereby affecting the sensitivity of bioluminescence imaging.
Application
The reaction of this enzyme with luciferin, ATP, and O2 results in the emission of light. Luciferase can be used to detect trace amounts of ATP. Firefly luciferase is also one of the most commonly utilized reporter genes for the study of gene expression. The bioluminescent reaction catalyzed by luciferase is one of the most sensitive analytical tools for measuring gene expression. < or equal to one femtomole of ATP can be detected using 0.2 μg of luciferase.
规格
产品名称
荧光素酶 来源于萤火虫
英文别名
Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) | firefly luciferase | luciferase (firefly luciferin) | Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing) | firefly luciferin luciferase | Photinus pyralis luciferase
规格或纯度
EnzymoPure™, 重组, lyophilized powder,≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein
CAS编号和信息
61970-00-1
酶学委员会编号
1.13.12.7
分子类型
酶
储存与运输
浓度
lyophilized powder,≥2.0×10^11 RlU/mg protein，lyophilized powder approximately 20% protein
储存条件
-80℃储存
运输条件
特低温运输
单位定义
One light unit produces a biometer peak height equivalent to 0.02 μCi of 14C in PPO/POPOP cocktail. Light units measured in 50 μl assay mixture containing 5 pmol ATP and 7.5 nmol luciferin in Tris-glycine buffer, pH 7.6, at 25°C.
高级数据
质检证书(CoA,COO,BSE/TSE 和分析图谱)
C of A & Other Certificates(BSE/TSE, COO):
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| 批号(Lot Number) | 证书类型 | 货号 |
|---|---|---|
 C2306046 |
分析证书 | L465180 |
技术文档和文章
此产品的引用文献
溶液计算器
产品仅提供给经过验证的企业、机构和合格的专业人员用于研发。不适用于人类、动物、诊断或治疗。
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